skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: [Induction of polygalacturonases important in pathogenicity of Pseudomonas solanacearum]

Abstract

Recent studies on the importance of hydroxyproline-rich glycoproteins (HPRG's) in the nature and function of plant cell walls have led to the question as to whether proteolytic enzymes are also involved in tissue maceration and act in concert with other cell wall degrading enzymes in the process. The primary objective of this research was to determine whether proteolytic enzymes, in combination with other enzymes, are involved in the degradation of plant cell walls and thus may be essential for pathogenesis by certain soft rot bacteria. The proteolytic enzymes of Erwinia carotovora subsp.carotovora (Ecc) grown on various media were examined by isoelectrofocusing in polyacrylamide gels over a pH range of 3-10. In addition to the main protease present in culture filtrates, low concentrations of several other proteases were present in extracts from potato tubers infected by Ecc. These enzymes degraded gelatin, soluble collagen, and Hide Powder Azure, and showed weak activity on casein, but did not degrade insoluble collagen or elastin. Ecc proteases appear capable of degrading at least one type of cell wall protein in vitro, but we were unable to obtain evidence that these proteases can attack cell wall proteins in muro. The results indicate that some glycosidic alkali-more » labile bonds have to be broken befor Ecc proteases can degrade cell wall proteins. Thus, these proteases may play a role in cell wall degradation only when acting in concert with other enzymes that split glycosidic bonds.« less

Publication Date:
Research Org.:
Wisconsin Univ., Madison, WI (United States). Dept. of Plant Pathology
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6697475
Report Number(s):
DOE/ER/13717-4
ON: DE93011613
DOE Contract Number:  
FG02-87ER13717
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CELL WALL; ENZYMATIC HYDROLYSIS; PEPTIDE HYDROLASES; ENZYME ACTIVITY; POTATOES; PSEUDOMONAS; BACTERIA; CELL CONSTITUENTS; CHEMICAL REACTIONS; DECOMPOSITION; ENZYMES; FOOD; HYDROLASES; HYDROLYSIS; LYSIS; MICROORGANISMS; ORGANIC COMPOUNDS; PLANTS; PROTEINS; SOLVOLYSIS; TUBERS; VEGETABLES; 090900* - Biomass Fuels- Processing- (1990-); 550500 - Metabolism; 550200 - Biochemistry

Citation Formats

. [Induction of polygalacturonases important in pathogenicity of Pseudomonas solanacearum]. United States: N. p., 1992. Web. doi:10.2172/6697475.
. [Induction of polygalacturonases important in pathogenicity of Pseudomonas solanacearum]. United States. https://doi.org/10.2172/6697475
. 1992. "[Induction of polygalacturonases important in pathogenicity of Pseudomonas solanacearum]". United States. https://doi.org/10.2172/6697475. https://www.osti.gov/servlets/purl/6697475.
@article{osti_6697475,
title = {[Induction of polygalacturonases important in pathogenicity of Pseudomonas solanacearum]},
author = {},
abstractNote = {Recent studies on the importance of hydroxyproline-rich glycoproteins (HPRG's) in the nature and function of plant cell walls have led to the question as to whether proteolytic enzymes are also involved in tissue maceration and act in concert with other cell wall degrading enzymes in the process. The primary objective of this research was to determine whether proteolytic enzymes, in combination with other enzymes, are involved in the degradation of plant cell walls and thus may be essential for pathogenesis by certain soft rot bacteria. The proteolytic enzymes of Erwinia carotovora subsp.carotovora (Ecc) grown on various media were examined by isoelectrofocusing in polyacrylamide gels over a pH range of 3-10. In addition to the main protease present in culture filtrates, low concentrations of several other proteases were present in extracts from potato tubers infected by Ecc. These enzymes degraded gelatin, soluble collagen, and Hide Powder Azure, and showed weak activity on casein, but did not degrade insoluble collagen or elastin. Ecc proteases appear capable of degrading at least one type of cell wall protein in vitro, but we were unable to obtain evidence that these proteases can attack cell wall proteins in muro. The results indicate that some glycosidic alkali- labile bonds have to be broken befor Ecc proteases can degrade cell wall proteins. Thus, these proteases may play a role in cell wall degradation only when acting in concert with other enzymes that split glycosidic bonds.},
doi = {10.2172/6697475},
url = {https://www.osti.gov/biblio/6697475}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1992},
month = {Wed Jan 01 00:00:00 EST 1992}
}