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Title: Survival and distribution of Vibrio cholerae in a tropical rain forest stream

Abstract

For 12 months Vibrio cholerae and fecal coliforms were monitored along with 9 other water quality parameters at 12 sites in a rain forest watershed in Puerto Rico. Densities of V. cholerae and fecal coliforms were not significantly correlated even though the highest densities of both bacteria were found at a sewage outfall. High densities of V. cholerae were also found at pristine sites high in the watershed. V. cholerae and Escherichia coli were inoculated into membrane diffusion chambers, placed at two sites and monitored for 5 days on two different occasions. Two different direct count methods indicated that the density of E. coli and V. cholerae did not change significantly during the course of either study. Physiological activity, as measured by INT-reduction and relative nucleic acid composition declined for E. coli during the first 12 h then increased and remained variable during the remainder of the study. V. cholerae activity, as measured by relative nucleic acid concentrations, remained high and unchanged for the entire study. INT-reduction in V. cholerae declined initially but regained nearly all of it`s original activity within 48 h. This study suggests that V. cholerae is an indigenous organism in tropical freshwaters and that assays othermore » than fecal coliforms or E. coli must be used for assessing public health risk in tropical waters.« less

Authors:
 [1];  [2]
  1. Univ. of Puerto Rico, Rio Piedras (Puerto Rico). Microbial Ecology Lab.
  2. E.I. DuPont de Nemours and Co., Aiken, SC (United States). Savannah River Lab.
Publication Date:
Research Org.:
E.I. DuPont de Nemours and Co., Savannah River Lab., Aiken, SC (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
OSTI Identifier:
666266
Report Number(s):
DP-MS-88-077
ON: DE98007473; CNN: Grant R/LR-08-87-THA1;Grant RR-2657;Grant RR-8102; TRN: AHC29819%%426
DOE Contract Number:  
AC09-89SR18035; AC09-76SR00001
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1988]
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; WATER POLLUTION; TROPICAL REGIONS; STREAMS; FECES; POPULATION DENSITY; NUMERICAL DATA; ESCHERICHIA COLI; BIOLOGICAL INDICATORS; BACTERIA; SURVIVAL TIME

Citation Formats

Perez-Rosas, N, and Hazen, T C. Survival and distribution of Vibrio cholerae in a tropical rain forest stream. United States: N. p., 1988. Web. doi:10.2172/666266.
Perez-Rosas, N, & Hazen, T C. Survival and distribution of Vibrio cholerae in a tropical rain forest stream. United States. https://doi.org/10.2172/666266
Perez-Rosas, N, and Hazen, T C. 1988. "Survival and distribution of Vibrio cholerae in a tropical rain forest stream". United States. https://doi.org/10.2172/666266. https://www.osti.gov/servlets/purl/666266.
@article{osti_666266,
title = {Survival and distribution of Vibrio cholerae in a tropical rain forest stream},
author = {Perez-Rosas, N and Hazen, T C},
abstractNote = {For 12 months Vibrio cholerae and fecal coliforms were monitored along with 9 other water quality parameters at 12 sites in a rain forest watershed in Puerto Rico. Densities of V. cholerae and fecal coliforms were not significantly correlated even though the highest densities of both bacteria were found at a sewage outfall. High densities of V. cholerae were also found at pristine sites high in the watershed. V. cholerae and Escherichia coli were inoculated into membrane diffusion chambers, placed at two sites and monitored for 5 days on two different occasions. Two different direct count methods indicated that the density of E. coli and V. cholerae did not change significantly during the course of either study. Physiological activity, as measured by INT-reduction and relative nucleic acid composition declined for E. coli during the first 12 h then increased and remained variable during the remainder of the study. V. cholerae activity, as measured by relative nucleic acid concentrations, remained high and unchanged for the entire study. INT-reduction in V. cholerae declined initially but regained nearly all of it`s original activity within 48 h. This study suggests that V. cholerae is an indigenous organism in tropical freshwaters and that assays other than fecal coliforms or E. coli must be used for assessing public health risk in tropical waters.},
doi = {10.2172/666266},
url = {https://www.osti.gov/biblio/666266}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1988},
month = {Sat Dec 31 00:00:00 EST 1988}
}