Flow cytometry-based DNA hybridization and polymorphism analysis
Functional analysis of the humane genome, including the quantification of differential gene expression and the identification of polymorphic sites and disease genes, is an important element of the Human Genome Project. Current methods of analysis are mainly gel-based assays that are not well-suited to rapid genome-scale analyses. To analyze DNA sequence on a large scale, robust and high throughput assays are needed. The authors are developing a suite of microsphere-based approaches employing fluorescence detection to screen and analyze genomic sequence. The approaches include competitive DNA hybridization to measure DNA or RNA targets in unknown samples, and oligo ligation or extension assays to analyze single-nucleotide polymorphisms. Apart from the advances of sensitivity, simplicity, and low sample consumption, these flow cytometric approaches have the potential for high throughput multiplexed analysis using multicolored microspheres and automated sample handling.
- Research Organization:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- National Insts. of Health, Bethesda, MD (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 663513
- Report Number(s):
- LA-UR-98-284; CONF-980117-; ON: DE98003495; TRN: AHC29818%%350
- Resource Relation:
- Conference: BIOS `98: an international symposium on biomedical optics, San Jose, CA (United States), 24-30 Jan 1998; Other Information: PBD: [1998]
- Country of Publication:
- United States
- Language:
- English
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