[Collection of low copy number repeats for use as probes in human DNA Mapping]
To isolate novel families of repeated human sequences our basic plan is to (i) construct repeated sequence libraries, (ii) discard known repeats by hybridization and (iii) sort through the remaining clones to eliminate single copy sequences as well as known repeats that slipped through the first screening. During the previous grant period, we completed the library construction and initial screening. Six size selected sublibraries of renatured human DNA were constructed, e.g. sublibrary II contains 250 nt to 380 nt inserts. The released inserts were hybridized to Alu, L1, THE 1, [alpha] satellite and satellite M (which crosshybridizes to satellites I and H) hybridization probes. We have adopted two strategies to sort through these candidate clones.
- Research Organization:
- California Univ., Davis, CA (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG03-88ER60693
- OSTI ID:
- 6613443
- Report Number(s):
- DOE/ER/60693-T3; ON: DE93013245
- Country of Publication:
- United States
- Language:
- English
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