Biochemical and molecular analysis of a transmembrane protein kinase from Arabidopsis thaliana

Technical Report · Fri Jan 01 00:00:00 EST 1993

DOI:https://doi.org/10.2172/6592071· OSTI ID:6592071

Bleecker, A B

We have isolated genomic and cDNA clones encoding a novel receptor-like protein kinase from the higher plant Arabidopsis thaliana. This kinase is being studied by combining biochemical, molecular, and genetic approaches. Domain-specific antibodies immunodecorate a polypeptide with a molecular mass of 120,000 daltons in extracts of Arabidopsis, where it has been found in all portions of the plant examined including root, stem, leaf, flower, and silique. Cytochemical analysis and initial studies using the kinase promoter with the GUS reporter gene system also indicate that the kinase is present throughout the plant. The kinase is glycosylated, like animal receptor kinases, and has been partially purified from Arabidopsis by using lectin columns. The kinase has been expressed in E coli, purified, and found to autophosphorylate on serine and threonine residues, but not on tyrosine residues. As such, it belongs to the small family of receptor-like kinases with serine/threonine specificity. Transgenic plants are now being produced that either overexpress or carry altered forms of the protein kinase gene. These experiments will help determine the natural role the kinase plays in a pathway of signal transduction.

• Research Organization: Wisconsin Univ., Madison, WI (United States). Dept. of Botany
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER20029
• OSTI ID: 6592071
• Report Number(s): DOE/ER/20029-1; ON: DE93014085
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
ESCHERICHIA COLI
GENETIC ENGINEERING
PHOSPHOTRANSFERASES
DNA-CLONING
ARABIDOPSIS
BIOTECHNOLOGY
PROGRESS REPORT
RECEPTORS
BACTERIA
CLONING
DNA HYBRIDIZATION
DOCUMENT TYPES
ENZYMES
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
PROTEINS
TRANSFERASES
090900* - Biomass Fuels- Processing- (1990-)
550200 - Biochemistry