ESR (electron spin resonance)-determined osmotic behavior of bull spermatozoa

Conference · Mon Jan 01 00:00:00 EST 1990

OSTI ID:6443789

Du, J; Kleinhans, F W; Spitzer, V J; Critser, J K ^[1]; Horstman, L ^[2]; Mazur, P ^[3]

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1. Methodist Hospital, Indianapolis, IN (USA). Dept. of Medical Research
2. Purdue Univ., Lafayette, IN (USA). School of Veterinary Medicine
3. Oak Ridge National Lab., TN (USA)

Our laboratories are pursuing a fundamental approach to the problems of semen cryopreservation. For many cell types (human red cells, yeast, HeLa) it has been demonstrated that there is an optimum cooling rate for cryopreservation. Faster rates allow insufficient time for cell dehydration and result in intracellular ice formation and cell death. It is possible to predict this optimal rate provided that the cell acts as an ideal osmometer and several other cell parameters are known such as the membrane hydraulic conductivity. It is the purpose of this work to examine the osmotic response of bull sperm to sucrose and NaCl utilizing electron spin resonance (ESR) to measure cell volume. For calibration purposes we also measured the ESR response of human red cells (RBC), the osmotic response of which is well documented with other methods. 15 refs., 1 fig.

• Research Organization: Oak Ridge National Lab., TN (USA)
• Sponsoring Organization: DOA; DOE/ER
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6443789
• Report Number(s): CONF-9008158-2; ON: DE91000728; CNN: 89-37240-4681
• Resource Relation: Conference: 2. international conference on the preservation of boar semen, Beltsville, MD (USA), 19-22 Aug 1990
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CRYOGENICS
SPERMATOZOA
CRYOBIOLOGY
BIOLOGICAL EFFECTS
CATTLE
CELL KILLING
CELL MEMBRANES
ELECTRON SPIN RESONANCE
ERYTHROCYTES
OSMOSIS
SACCHAROSE
SODIUM CHLORIDES
ALKALI METAL COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CHLORIDES
CHLORINE COMPOUNDS
DIFFUSION
DISACCHARIDES
DOMESTIC ANIMALS
GAMETES
GERM CELLS
HALIDES
HALOGEN COMPOUNDS
MAGNETIC RESONANCE
MAMMALS
MATERIALS
MEMBRANES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
RESONANCE
RUMINANTS
SACCHARIDES
SODIUM COMPOUNDS
VERTEBRATES
553000* - Agriculture & Food Technology
560200 - Thermal Effects