Biological (molecular and cellular) markers of toxicity

Technical Report · Mon Oct 01 00:00:00 EDT 1990

DOI:https://doi.org/10.2172/6441197· OSTI ID:6441197

Shugart, L R

The overall objective of this study is to evaluate the use of the small aquarium fish, Japanese Medaka (Oryzias latipes), as a predictor of potential genotoxicity following exposure to carcinogens. This will be accomplished by quantitatively investigating the early molecular events associated with genotoxicity of various tissues of Medaka subsequent to exposure of the organism to several known carcinogens, such as diethylnitrosamine (DEN) and benzo(a)pyrene (BaP). Because of the often long latent period between initial contact with certain chemical and physical agents in our environment and subsequent expression of deleterious health or ecological impact, the development of sensitive methods for detecting and estimating early exposure is needed so that necessary interventions can ensue. A promising biological endpoint for detecting early exposure to damaging chemicals is the interaction of these compounds with cellular macromolecules such as Deoxyribonucleic acids (DNA). This biological endpoint assumes significance because it can be one of the critical early events leading eventually to adverse effects (neoplasia) in the exposed organism.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOD
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6441197
• Report Number(s): ORNL/M-1305; ON: DE91004700
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ACETYLAMINOFLUORENES
TOXICITY
AMINES
BENZOPYRENE
FISHES
NEOPLASMS
BIOLOGICAL MARKERS
BIOASSAY
CARCINOGEN SCREENING
CARCINOGENS
DETOXIFICATION
DNA
DNA REPAIR
ENZYME ACTIVITY
LIVER
MUTAGEN SCREENING
MUTAGENS
PROGRESS REPORT
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DISEASES
DOCUMENT TYPES
GLANDS
HYDROCARBONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RECOVERY
REPAIR
SCREENING
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry