Calmodulin immunolocalization to cortical microtubules is calcium independent
Calcium affects the stability of cortical microtubules (MTs) in lysed protoplasts. This calmodulin (CaM)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with MT function. To test the hypothesis that CaM associates with these MTs, monoclonal antibodies were produced against CaM, and one (designated mAb1D10), was selected for its suitability as an immunocytochemical reagent. It is shown that CaM associates with the cortical Mats of cultured carrot (Daucus carota L.) and tobacco (Nicotiana tobacum L.) cells. Inasmuch as CaM interacts with calcium and affects the behavior of these Mats, we hypothesized that calcium would alter this association. To test this, protoplasts containing taxol-stabilized Mats were lysed in the presence of various concentrations of calcium and examined for the association of Cam with cortical Mats. At 1 [mu]M calcium, many protoplasts did not have CaM in association with the cortical Mats, while at 3.6 [mu]M calcium, this association was completely abolished. The results are discussed in terms of a model in which CaM associates with Mats via two types of interactions; one calcium dependent and one independent.
- Research Organization:
- Pennsylvania State Univ., University Park, PA (United States). Dept. of Biology
- Sponsoring Organization:
- USDOE; DOA; USDOE, Washington, DC (United States); Department of Agriculture, Washington, DC (United States)
- DOE Contract Number:
- FG02-91ER20050
- OSTI ID:
- 6434308
- Report Number(s):
- DOE/ER/20050-2; ON: DE93015098
- Country of Publication:
- United States
- Language:
- English
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CALCIUM
MOLECULAR BIOLOGY
CALMODULIN
BIOLOGICAL FUNCTIONS
MICROTUBULES
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
HYBRIDOMAS
NICOTIANA
PLANT CELLS
ALKALINE EARTH METALS
ANIMAL CELLS
CELL CONSTITUENTS
ELEMENTS
KINETICS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
REACTION KINETICS
550200* - Biochemistry
550300 - Cytology