Effects of calmodulin inhibitors on the cellular metabolism of /sup 45/Ca and /sup 210/Pb
Altered Ca/sup + +/ homeostasis may result from the direct inhibition of calmodulin-dependent or -independent transport processes. Changes in cell function not directly related to the transport of Ca, e.g., uncoupling of oxidative phosphorylation or altered membrane permeability also disrupt cell calcium metabolism. Thus, the effects of the calmodulin inhibitor W-13 on cell Pb/sup + +/ metabolism may be due to its direct effects on Pb/sup + +/ transporting Ca/sup + +/ pumps, or indirectly as a result of changes in Ca/sup + +/ homeostasis. Direct comparison of the effects of W-13 on the metabolism of Pb and Ca is impaired by differences in the kinetic distribution and behavior of Pb and Ca. A further complication is that the calmodulin-dependent processes are most active during periods of elevated intracellular Ca/sup + +/. The preliminary experiments reported here were conducted in unstimulated cells which have a low resting level of cytosolic Ca/sup + +/. Therefore, W-13 induced alterations in cell Ca/sup + +/ and Pb/sup + +/ may not reflect the changes which could occur in stimulated cells. 1 ref., 1 fig.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 6408053
- Report Number(s):
- BNL-39866; CONF-8705124-1; ON: DE87010790
- Resource Relation:
- Conference: TEMA-6: 6th international conference on trace elements in man and animals, Asilomar, CA, USA, 31 May 1987; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CALCIUM 45
MEMBRANE TRANSPORT
CALMODULIN
BIOCHEMICAL REACTION KINETICS
LEAD 210
BIOLOGICAL EFFECTS
ENZYME INHIBITORS
RECEPTORS
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALPHA DECAY RADIOISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
HEAVY NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEAD ISOTOPES
MEMBRANE PROTEINS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
YEARS LIVING RADIOISOTOPES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques
550201 - Biochemistry- Tracer Techniques