DNA analysis of epithelial cell suspensions
Cell suspensions of skin were obtained by animals exposed by skin painting of several crude oils. DNA analysis of these cell suspensions labeled with mithramycin provide determination of percentages of cells in the G/sub 1/, S and G/sub 2/M phases of the cell cycle. Data acquired showed differences from control animals occurring as early as 7 days after treatment and persisting through 21 days afterwards. There was histological evidence of erythema and hyperplasia in shale oil-exposed skins. Flow cytometric analysis of DNA content in shale-oil-exposed skin cells showed an increased percentage of cycling cells plus evidence of aneuploidy. Similar data from simply abraded skin showed increased percentages of cycling cells, but no aneuploidy. The shale-oil-exposed group, when compared to a standard petroleum-exposed group, had significantly increased percentages of cycling cells. This early indication of differing response to different complex mixtures was also seen in long-term skin exposures to these compounds. Similar analytical techniques were applied to tracheal cell suspensions from ozone-exposed rats. 12 refs., 4 figs., 4 tabs. (DT)
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6391489
- Report Number(s):
- LA-UR-85-3568; CONF-8510225-1; ON: DE86002403
- Resource Relation:
- Conference: Hanford life sciences symposium, Richland, WA, USA, 21 Oct 1985
- Country of Publication:
- United States
- Language:
- English
Similar Records
Flow cytometric analysis of respiratory tract cells exposed to oil shale and silica particulates. [Hamsters]
Detection of early changes in lung cell cytology by flow-systems analysis techniques. Progress report, January 1-June 30, 1979
Related Subjects
04 OIL SHALES AND TAR SANDS
EPITHELIUM
CELL PROLIFERATION
OZONE
BIOLOGICAL EFFECTS
SHALE OIL
ANTINEOPLASTIC DRUGS
CELL CULTURES
CELL FLOW SYSTEMS
DNA
EXPERIMENTAL DATA
SKIN
TRACHEA
ANIMAL TISSUES
BODY
DATA
DRUGS
ENERGY SOURCES
FOSSIL FUELS
FUELS
INFORMATION
MINERAL OILS
NUCLEIC ACIDS
NUMERICAL DATA
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
RESPIRATORY SYSTEM
TISSUES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
040700 - Oil Shales & Tar Sands- Health & Safety