Bioluminescent reporters for catabolic gene expression and pollutant bioavailability

Conference · Tue Jan 01 00:00:00 EST 1991

OSTI ID:6390215

Heitzer, A; DiGrazia, P M; Sayler, G S ^[1]; Burlage, R S ^[2]

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1. Tennessee Univ., Knoxville, TN (United States). Center for Environmental Biotechnology
2. Oak Ridge National Lab., TN (United States)

The application of visualized catabolic nah-gene expression using a luxCDABE gene fusion provides a valuable method to measure quantitatively and specifically naphthalene and salicylate bioavailability. It has been demonstrated that the physiological state of the test culture together with the intrinsic regulation mechanisms of the naphthalene degradation pathway as well as the physiological aspects of the lux gene fusion have to be taken into account. The method presented provides a high potential for in situ bioprocess monitoring. In addition, the results obtained with immobilized cells provide a basis for the development of biosensors for environmental applications in specific pollutant monitoring in waste streams and soil slurry systems but, as a general method, also for more conventional biotechnological process control. 8 refs., 2 figs., 1 tab.

• Research Organization: Oak Ridge National Lab., TN (United States)
• Sponsoring Organization: USDOE; USDOD; USDOE, Washington, DC (United States); Department of Defense, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400; FG05-91ER61193
• OSTI ID: 6390215
• Report Number(s): CONF-9110267-1; ON: DE92002687; CNN: F49620-89-C-0023
• Resource Relation: Conference: International marine biotechnology conference, Baltimore, MD (United States), 17-18 Oct 1991
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
54 ENVIRONMENTAL SCIENCES
CATABOLISM
GENE REGULATION
MONITORING
NAPHTHALENE
BIODEGRADATION
BIOLOGICAL AVAILABILITY
SALICYLIC ACID
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BIOLUMINESCENCE
BIOTECHNOLOGY
GENE OPERONS
GENETIC ENGINEERING
IMMOBILIZED CELLS
INFORMATION NEEDS
MOLECULAR BIOLOGY
PSEUDOMONAS
SOILS
TRANSCRIPTION
AROMATICS
BACTERIA
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
CONDENSED AROMATICS
DECOMPOSITION
HYDROCARBONS
HYDROXY ACIDS
LUMINESCENCE
METABOLISM
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
560300* - Chemicals Metabolism & Toxicology
540220 - Environment
Terrestrial- Chemicals Monitoring & Transport- (1990-)
540320 - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)