Labeling cellular elements of blood with Technetium-99m
The purpose of this proposal is to develop new technique of labeling platelets and white cells with Tc-99m radionuclide. The conditions of labeling canine platelets and white cells with the lipid-soluble Tc-99m HMPAO have been optimized. The function of labeled platelets were evaluated by the determination of platelet survival time and recovery and these values were compared with that of In-111 tropolone labeled platelets. We developed the bilateral femoral catheterization model for the evaluation of platelet-thrombosis on control and heparin-bonded catheters in dogs. We are evaluating platelet thrombosis in the hollow-fiber hemodialyzer with Tc-99m and In-111 labeled platelets. We have developed the flow-loop for in vitro studies and are using a pig model for quantitation of platelet-consumption during hemodialysis. We are currently evaluating the new technique of platelet and white cell-labeling with Tc-99m and testing them in animal models of thrombosis and infection (osteo-myelitis). We are also using the Tc-99m HMPAO labeled mixed white cells in the early diagnosis (3-hour post-injection) of acute and chronic infection in patients and comparing the results with that of IN-111 oxine labeled white cells.
- Research Organization:
- Miami Univ., FL (USA). School of Medicine
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG05-88ER60728
- OSTI ID:
- 6366396
- Report Number(s):
- DOE/ER/60728-2; ON: DE91002371
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
LABELLING
LEUKOCYTES
TECHNETIUM 99
BIOLOGICAL EFFECTS
DIAGNOSTIC TECHNIQUES
DIALYSIS
DOGS
HEPARIN
INDIUM 111
PROGRESS REPORT
SURVIVAL TIME
THROMBOSIS
TRACER TECHNIQUES
URINE
AMINES
ANIMALS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DISEASES
DOCUMENT TYPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HEMATOLOGIC AGENTS
HOURS LIVING RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SEPARATION PROCESSES
TECHNETIUM ISOTOPES
VASCULAR DISEASES
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques