Phytochromes in photosynthetically competent plants
Major improvements have been made in the purification of green-oat phytochrome. An effective protease inhibitor has been incorporated, the scale of preparations has been increased greatly, an immunodominant contaminant has been eliminated, and the extent of purification has been increased by at least a factor of ten. Five new MAbs and rabbit PAbs to green-oat phytochrome, as well as rabbit PAbs to a synthetic, putative green-oat phytochrome peptide, have been produced and characterized, together with two MAbs to green-oat phytochrome that had been identified previously. Our earlier hypothesis that green-oat phytochrome itself consists of two types was found to be true. One type of green-oat phytochrome has an apparent monomer size of 125 kDa while the other is 123 kDa. The latter undergoes a Zn[sup 2+]-induced mobility shift during SDS PAGE and the two phytochromes are immunochemically distinct from one another. Affinity columns prepared with MAbs to green-oat phytochrome have been used to purify 125-kDa green-oat phytochrome to near homogeneity. A proteolytically derived peptide has been isolated from immunopurified green-oat phytochrome and 19 residues have been determined by microsequencing. The results verify that in monocotyledons as well as dicotyledons green- and etiolated-oat phytochromes derive from different genes.
- Research Organization:
- Georgia Univ., Athens, GA (United States). Dept. of Botany
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG09-87ER13709
- OSTI ID:
- 6239721
- Report Number(s):
- DOE/ER/13709-5; ON: DE93015938
- Country of Publication:
- United States
- Language:
- English
Similar Records
Phytochrome from green plants: assay, purification and characterization. Progress report, June 1, 1984-July 15, 1986
Phytochrome in photosynthetically competent plants: characterization by monoclonal antibodies. Progress report
Related Subjects
14 SOLAR ENERGY
60 APPLIED LIFE SCIENCES
PHYTOCHROMES
FRACTIONATION
ABSORPTION SPECTRA
AMINO ACID SEQUENCE
HYBRIDOMAS
IMMUNOASSAY
MONOCLONAL ANTIBODIES
OATS
PROGRESS REPORT
ANIMAL CELLS
ANTIBODIES
BIOASSAY
CEREALS
DOCUMENT TYPES
GRAMINEAE
LILIOPSIDA
MAGNOLIOPHYTA
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PIGMENTS
PLANTS
PROTEINS
SEPARATION PROCESSES
SPECTRA
550200* - Biochemistry
140505 - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)
553000 - Agriculture & Food Technology