Genotoxicity of complex mixtures: CHO cell mutagenicity assay
A Chinese hamster ovary (CHO) mammalian cell assay was used to evaluate the genotoxicity of complex mixtures (synthetic fuels). The genotoxicity (mutagenic potency) of the mixtures increased as the temperature of their boiling range increased. Most of the genotoxicity in the 750/sup 0/F+ boiling-range materials was associated with the neutral polycyclic aromatic hydrocarbon (PAH) fractions. Chemical analysis data indicate that the PAH fractions of high-boiling coal liquids contain a number of known chemical carcinogens, including five- and six-ring polyaromatics (e.g., benzo(a)pyrene) as well as four- and five-ring alkyl-substituted PAH (e.g., methylchrysene and dimethylbenzanthracenes); concentrations are a function of boiling point (bp). In vitro genotoxicity was also detected in fractions of nitrogen-containing polyaromatic compounds, as well as in those with aliphatics of hydroxy-containing PAH. Mutagenic activity of some fractions was detectable in the CHO assay in the absence of an exogenous metabolic activation system; in some instances, addition of exogenous enzymes and cofactors inhibited expression of the direct-acting mutagenic potential of the fraction. These data indicate that the organic matrix of the chemical fraction determines whether, and to what degree, various mutagens are expressed in the CHO assay. Therefore, the results of biological assays of these mixtures must be correlated with chemical analyses for proper interpretation of these data. 29 references, 16 figures, 4 tables.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 6135285
- Report Number(s):
- PNL-5337; ON: DE85008354
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYCYCLIC AROMATIC HYDROCARBONS
MUTAGEN SCREENING
SYNTHETIC FUELS
BENZOPYRENE
BOILING POINTS
CHO CELLS
DIMETHYLBENZANTHRACENE
EXPERIMENTAL DATA
MUTAGENESIS
TEMPERATURE DEPENDENCE
TOXICITY
ANIMAL CELLS
AROMATICS
CONDENSED AROMATICS
DATA
FUELS
HYDROCARBONS
INFORMATION
NUMERICAL DATA
ORGANIC COMPOUNDS
PHYSICAL PROPERTIES
SCREENING
THERMODYNAMIC PROPERTIES
TRANSITION TEMPERATURE
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)