Pathway engineering to improve ethanol production by thermophilic bacteria
Continuation of a research project jointly funded by the NSF and DOE is proposed. The primary project goal is to develop and characterize strains of C. thermocellum and C. thermosaccharolyticum having ethanol selectivity similar to more convenient ethanol-producing organisms. An additional goal is to document the maximum concentration of ethanol that can be produced by thermophiles. These goals build on results from the previous project, including development of most of the genetic tools required for pathway engineering in the target organisms. As well, we demonstrated that the tolerance of C. thermosaccharolyticum to added ethanol is sufficiently high to allow practical utilization should similar tolerance to produced ethanol be demonstrated, and that inhibition by neutralizing agents may explain the limited concentrations of ethanol produced in studies to date. Task 1 involves optimization of electrotransformation, using either modified conditions or alternative plasmids to improve upon the low but reproducible transformation, frequencies we have obtained thus far.
- Research Organization:
- Dartmouth Coll., Hanover, NH (United States). Thayer School of Engineering
- Sponsoring Organization:
- USDOE Assistant Secretary for Energy Efficiency and Renewable Energy, Washington, DC (United States); National Science Foundation, Washington, DC (United States)
- DOE Contract Number:
- AI01-93CE50364
- OSTI ID:
- 576095
- Report Number(s):
- DOE/CE/50364-T1; ON: DE98000723; CNN: BCS9215130; BCS9314449; TRN: 98:001415
- Resource Relation:
- Other Information: PBD: [1998]
- Country of Publication:
- United States
- Language:
- English
Similar Records
Production of ethanol from lignocellulosic materials using thermophilic bacteria
Pathway engineering and organism development for ethanol production from cellulosic biomass using thermophilic bacteria