Measurement of background translocation frequencies in individuals with clones
Abstract
In the leukemia case the unseparated B and T lymphocytes had a high translocation frequency even after 0.0014, respectively. After purging all clones from the data, the translocation frequencies for Bio 8 and Bio 23 were 0.00750.0014 and 0.0073 metaphases were scored for chromosomal aberrations,, specifically reciprocal translocations, using fluorescence in situ hybridization (FISH). Metaphase spreads were used from two healthy, unexposed individuals (not exposed to radiation, chemotherapy or radiotherapy) and one early B- precursor acute lymphocytic leukemia (ALL) patient (metaphase spreads from both separated T lymphocytes and unseparated B and T lymphocytes were scored). All three individuals had an abnormally high translocation frequency. The high translocation frequencies resulted from clonal expansion of specific translocated chromosomes. I show in this thesis that by purging (discounting or removing) clones from the data of unexposed individuals, one can obtain true background translocation frequencies. In two cases, Bio 8 and Bio 23, the measured translocation frequency for chromosomes 1, 2 and 4 was 0.0124 purging all of the clones from the data. This high translocation frequency may be due to a low frequency of some clones and may not be recognized. The separated T lymphocytes had a higher translocation frequency than expected.
- Authors:
-
- California State Univ. (CalState), Hayward, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 572769
- Report Number(s):
- UCRL-LR-125903
ON: DE98050671
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Thesis/Dissertation
- Resource Relation:
- Other Information: TH: MS; PBD: 1 Aug 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; MAN; GENETIC VARIABILITY; RFLPS; BIOLOGICAL VARIABILITY; CHROMOSOMAL ABERRATIONS; GENE RECOMBINATION
Citation Formats
Wade, Marcelle J. Measurement of background translocation frequencies in individuals with clones. United States: N. p., 1996.
Web. doi:10.2172/572769.
Wade, Marcelle J. Measurement of background translocation frequencies in individuals with clones. United States. https://doi.org/10.2172/572769
Wade, Marcelle J. 1996.
"Measurement of background translocation frequencies in individuals with clones". United States. https://doi.org/10.2172/572769. https://www.osti.gov/servlets/purl/572769.
@article{osti_572769,
title = {Measurement of background translocation frequencies in individuals with clones},
author = {Wade, Marcelle J.},
abstractNote = {In the leukemia case the unseparated B and T lymphocytes had a high translocation frequency even after 0.0014, respectively. After purging all clones from the data, the translocation frequencies for Bio 8 and Bio 23 were 0.00750.0014 and 0.0073 metaphases were scored for chromosomal aberrations,, specifically reciprocal translocations, using fluorescence in situ hybridization (FISH). Metaphase spreads were used from two healthy, unexposed individuals (not exposed to radiation, chemotherapy or radiotherapy) and one early B- precursor acute lymphocytic leukemia (ALL) patient (metaphase spreads from both separated T lymphocytes and unseparated B and T lymphocytes were scored). All three individuals had an abnormally high translocation frequency. The high translocation frequencies resulted from clonal expansion of specific translocated chromosomes. I show in this thesis that by purging (discounting or removing) clones from the data of unexposed individuals, one can obtain true background translocation frequencies. In two cases, Bio 8 and Bio 23, the measured translocation frequency for chromosomes 1, 2 and 4 was 0.0124 purging all of the clones from the data. This high translocation frequency may be due to a low frequency of some clones and may not be recognized. The separated T lymphocytes had a higher translocation frequency than expected.},
doi = {10.2172/572769},
url = {https://www.osti.gov/biblio/572769},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Aug 01 00:00:00 EDT 1996},
month = {Thu Aug 01 00:00:00 EDT 1996}
}