Biophysical and biological factors determining the ability to achieve long-term cryobiological preservation
- Oak Ridge National Lab., TN (United States). Life Sciences Div.
The BESTCapsule will maintain appropriate biological specimens for decades or centuries at cryogenic temperatures in the living state. Maintenance at temperatures below {approximately} {minus}140 C is not a problem. No ordinary chemical reactions in aqueous solutions can occur. The only source of damage will be the slow accumulation of physical damage to DNA from background ionizing radiation. But this source of damage should not become serious in less than a millennium. Rather, the main problem in cryopreservation is to devise procedures for cooling the biological specimens to {minus}196 C and returning them to normal temperatures without inflicting lethal injury. Regardless of the cell type, there are certain encompassing biophysical factors and constraints that determine whether they will survive or die during freezing and thawing. Superimposed on these may be special biological factors that apply to specific cell types. This paper will emphasize the former and give illustrative examples of the latter.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States); National Science Foundation, Washington, DC (United States)
- DOE Contract Number:
- AC05-96OR22464
- OSTI ID:
- 554808
- Report Number(s):
- ORNL/CP-95102; CONF-971178-; ON: DE98001256; BR: KP0403000; CNN: Grant RO1-HD 30274; TRN: AHC29801%%70
- Resource Relation:
- Conference: BESTCapsule 2001 workshop, Osaka (Japan), 2-6 Nov 1997; Other Information: PBD: [1997]
- Country of Publication:
- United States
- Language:
- English
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