Enzymology of acetone-butanol-isopropanol formation

Technical Report · Wed Jan 01 00:00:00 EST 1992

DOI:https://doi.org/10.2172/5531414· OSTI ID:5531414

Chen, Jiann-Shin

The long-term goal of the project is to understand the fundamental properties of biological solvent production. Our approach is to elucidate first the molecular properties of solvent-producing enzymes and then to apply to information gained from the enzymological study to investigate control mechanisms for the solvent-producing pathways and the expression of solvent-production genes. Our research primarily involves two strains of Clostridium beijerinckii: C. Beijerinckii NRRL B593 which produces isopropanol in addition to acetone, butanol, and ethanol, and C. beijerinckii NRRL B592 which produces acetone, butanol and ethanol, but not isopropanol. In more recent studies, we also included another solvent-producing organism, Bacillus macerans. Objectives for the reporting period were: to characterize the distinct types of alcohol dehydrogenase; to purify and characterize acetoacetyl-CoA-reacting enzymes; and to clone and sequence the gene encoding the primary/secondary alcohol dehydrogenase of C beijerinckii NRRL B593 and to search for the promoter region for solvent-production genes.

• Research Organization: Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-85ER13368
• OSTI ID: 5531414
• Report Number(s): DOE/ER/13368-8; ON: DE92007704
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACETONE
SYNTHESIS
BUTANOLS
CLOSTRIDIUM
GENES
PROPANOLS
CLONING
ENZYMES
OXIDOREDUCTASES
PROGRESS REPORT
SOLVENTS
ALCOHOLS
BACTERIA
DOCUMENT TYPES
HYDROXY COMPOUNDS
KETONES
MICROORGANISMS
ORGANIC COMPOUNDS
PROTEINS
550700* - Microbiology
550400 - Genetics
550200 - Biochemistry