An improved method for producing radiation hybrids applied to human chromosome 19

Technical Report · Wed Jan 01 00:00:00 EST 1992

DOI:https://doi.org/10.2172/5453140· OSTI ID:5453140

Jackson, C L

At the initiation of the grant we had just produced radiation hybrids from a monochromosomal microcell hybrid containing human chromosome 19 as its only human component. Radiation hybrids were produced using doses of radiation ranging from 1000--8000 rads. Lethally irradiated cells were then fused to hamster recipients (CHTG49) and selected for growth in histidinol. Approximately 240 clones were isolated and 75 clones were expanded for the isolation of DNA. This report describes in situ hybridization studies and the introduction of markers into human chromosome 19.

• Research Organization: Rhode Island Hospital, Providence, RI (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER61136
• OSTI ID: 5453140
• Report Number(s): DOE/ER/61136-1; ON: DE92011030
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HUMAN CHROMOSOME 19
DNA HYBRIDIZATION
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMES
PROGRESS REPORT
BIOLOGICAL EFFECTS
DOCUMENT TYPES
HUMAN CHROMOSOMES
HYBRIDIZATION
RADIATION EFFECTS
550400* - Genetics
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture