Environmental Sciences Division Toxicology Laboratory standard operating procedures

Technical Report · Fri Sep 01 00:00:00 EDT 1989

DOI:https://doi.org/10.2172/5400204· OSTI ID:5400204

Kszos, L. A.; Stewart, A. J.; Wicker, L. F.; Logsdon, G. M.

This document was developed to provide the personnel working in the Environmental Sciences Division's Toxicology Laboratory with documented methods for conducting toxicity tests. The document consists of two parts. The first part includes the standard operating procedures (SOPs) that are used by the laboratory in conducting toxicity tests. The second part includes reference procedures from the US Environmental Protection Agency document entitled Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, upon which the Toxicology Laboratory's SOPs are based. Five of the SOPs include procedures for preparing Ceriodaphnia survival and reproduction test. These SOPs include procedures for preparing Ceriodaphnia food (SOP-3), maintaining Ceriodaphnia cultures (SOP-4), conducting the toxicity test (SOP-13), analyzing the test data (SOP-13), and conducting a Ceriodaphnia reference test (SOP-15). Five additional SOPs relate specifically to the fathead minnow (Pimephales promelas) larval survival and growth test: methods for preparing fathead minnow larvae food (SOP-5), maintaining fathead minnow cultures (SOP-6), conducting the toxicity test (SOP-9), analyzing the test data (SOP-12), and conducting a fathead minnow reference test (DOP-14). The six remaining SOPs describe methods that are used with either or both tests: preparation of control/dilution water (SOP-1), washing of glassware (SOP-2), collection and handling of samples (SOP-7), preparation of samples (SOP-8), performance of chemical analyses (SOP-11), and data logging and care of technical notebooks (SOP-16).

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: DOE/DP
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5400204
• Report Number(s): ORNL/TM-11194; ON: DE90001849; TRN: 89-029035
• Resource Relation: Other Information: Environnmental Sciences Division Publication No. 3337
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
54 ENVIRONMENTAL SCIENCES
AQUATIC ORGANISMS
MORTALITY
CHEMICAL EFFLUENTS
TOXICITY
Y-12 PLANT
LIQUID CONTAMINATION MONITORS
COMPLIANCE
DATA ACQUISITION
DATA ANALYSIS
PROGRAM MANAGEMENT
RADIOACTIVE EFFLUENTS
REGULATIONS
STANDARDS
TESTING
MANAGEMENT
MATERIALS
MEASURING INSTRUMENTS
MONITORS
NATIONAL ORGANIZATIONS
RADIATION MONITORS
RADIOACTIVE MATERIALS
RADIOACTIVE WASTES
US AEC
US DOE
US ERDA
US ORGANIZATIONS
WASTES
560300* - Chemicals Metabolism & Toxicology
520200 - Environment
Aquatic- Chemicals Monitoring & Transport- (-1989)