Translocation of particles to the pleural space and tracheobronchial lymph nodes following lung deposition
The translocation of particles from the alveolar compartment to the pleural space and tracheobronchial lymph nodes was investigated in rats over a 30 day period following the intrapulmonary instillation of 4 x 10/sup 8/ polystyrene microspheres (1.9 ..mu..m dia.). In initial studies, approx. =10/sup 4/ particles were found in the pleural space compartment on Days 1, 14 and 30 after particle deposition, and most of these were cell-associated. Subsequent pleural space studies indicated, however, that the particles found in this compartment were, at least in part, due to the pleural lavage technique, and, unlike observations reported for some fibers, the translocation of the particles used in our study to the pleural space does not represent an important lung clearance pathway. In regard to particle clearance to the tracheobronchial lymph nodes, the accumulation of particles, most of which were extracellular, kinetically was biphasic with the most rapid phase occurring within the first 24 hours. Over the Day 1-30 period, the numbers of particles in the lymph nodes increased linearly to approx. =1.2 x 10/sup 6/ microspheres. The percentages of the nodal particles that were associated with nodal mononuclear phagocytes (NMP) over the course of the study were inversely proportional to nodal particulate burdens, even though the percentage of cells with engulfed particles increased; the percentage of NMP asymptotically approached a maximum value over the range of nodal burden of 6-12 x 10/sup 5/ particles. The distributions of the microspheres in the NMP were essentially identical on Days 1, 14, and 30. Major differences in the distributions of particles in lavage alveolar macrophages and NMP were not consistent with the notion that the latter represented translocated AM. 20 refs., 7 figs., 4 tabs.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5375760
- Report Number(s):
- LA-UR-85-2553; CONF-8509138-1; ON: DE85015695
- Resource Relation:
- Conference: 6. international symposium on inhaled particles, Cambridge, MA, USA, 1 Sep 1985
- Country of Publication:
- United States
- Language:
- English
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LUNG CLEARANCE
TIME DEPENDENCE
MICROSPHERES
CELL FLOW SYSTEMS
EXPERIMENTAL DATA
LEUKOCYTES
LYMPH NODES
MACROPHAGES
POLYSTYRENE
RATS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLEARANCE
CONNECTIVE TISSUE CELLS
DATA
EXCRETION
INFORMATION
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PHAGOCYTES
PLASTICS
POLYMERS
POLYOLEFINS
POLYVINYLS
RODENTS
SOMATIC CELLS
SYNTHETIC MATERIALS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)