Movable genetic elements: detection of changes in maize DNA at the Shrunken locus due to the intervention of Ds elements

Burr, B; Burr, F A

Title: Movable genetic elements: detection of changes in maize DNA at the Shrunken locus due to the intervention of Ds elements

Full Record
Other Related Research

Abstract

This report describes our initial attempts at the molecular characterization of a maize controlling element. We have prepared a cDNA probe and used it to detect changes at a locus where Ds elements are found. Evidence of their presence are indicated by changes in the restriction patterns, but there is as yet no information on the physical nature of the controlling elements nor on the kinds of rearrangements they cause.

Authors:: Burr, B; Burr, F A

Publication Date:: Wed May 28 00:00:00 EDT 1980

Research Org.:: Brookhaven National Lab., Upton, NY (USA)

Sponsoring Org.:: U.S. Department of Energy (DOE)

OSTI Identifier:: 5277334

Report Number(s):: BNL-28042; CONF-800595-1

DOE Contract Number:: AC02-76CH00016

Resource Type:: Conference

Resource Relation:: Conference: Cold Spring Harbor symposium No. 45: movable genetic elements, Cold Spring Harbor, NY, USA, 28 May 1980

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; GENES; MOLECULAR STRUCTURE; MAIZE; DNA; MUTANTS; CEREALS; GRAMINEAE; GRASS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; 550400 - Genetics; 550200 - Biochemistry; 550400* - Genetics

Citation Formats


                    Burr, B, and Burr, F A. Movable genetic elements: detection of changes in maize DNA at the Shrunken locus due to the intervention of Ds elements.  United States: N. p., 1980. 
        Web.

Copy to clipboard


                    Burr, B, & Burr, F A. Movable genetic elements: detection of changes in maize DNA at the Shrunken locus due to the intervention of Ds elements.  United States.

Copy to clipboard


                    Burr, B, and Burr, F A. 1980.  
        "Movable genetic elements: detection of changes in maize DNA at the Shrunken locus due to the intervention of Ds elements".  United States.  https://www.osti.gov/servlets/purl/5277334.

Copy to clipboard


                    
@article{osti_5277334,

  title        = {Movable genetic elements: detection of changes in maize DNA at the Shrunken locus due to the intervention of Ds elements},

  author       = {Burr, B and Burr, F A},

  abstractNote = {This report describes our initial attempts at the molecular characterization of a maize controlling element. We have prepared a cDNA probe and used it to detect changes at a locus where Ds elements are found. Evidence of their presence are indicated by changes in the restriction patterns, but there is as yet no information on the physical nature of the controlling elements nor on the kinds of rearrangements they cause.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5277334},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed May 28 00:00:00 EDT 1980},

  month        = {Wed May 28 00:00:00 EDT 1980}

}

Copy to clipboard

Conference:

View Conference (0.34 MB)

Other availability

Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Detection of changes in maize DNA at the shrunken locus due to the intervention of Ds elements

Journal Article Burr, B; Burr, F - Cold Spring Harbor Symp. Quant. Biol.; (United States)

This report describes our initial attempts at the molecular characterization of a controlling element in maize. A cDNA probe has been prepared to detect changes at a locus where Ds (Dissociation) elements are found. Evidence of their presence is indicated by changes in the restriction patterns, but there is as yet no information on the physical nature of the controlling elements or on the kinds of rearrangements they cause. It should be remembered that at the 1951 Cold Spring Harbor Symposium, McClintock (1952a) provided extensive evidence for the physical presence of controlling elements.
https://doi.org/10.1101/SQB.1981.045.01.061
Ds controlling elements of maize at the shrunken locus are large and dissimilar insertions

Journal Article Burr, B; Burr, F - Cell; (United States)

A recombinant clone for the wild-type gene of succrose synthetase has been selected. Subclones of the genomic clone were made and used as probes to construct a restriction map of the wild-type locus. the transcribed region and the direction of transcription in the map were also determined. Four Ds-induced mutations of the locus that were isolated by B. McClintock were analyzed and partially mapped. Two mutations contain insertions of approx. 20 kb of foreign DNA into the transcribed region; two others are at the 5' end of the gene. There are profound differences in the restriction maps of the Dsmore »« less
https://doi.org/10.1016/0092-8674(82)90461-5
Controlling-element events at the shrunken locus in maize

Journal Article Burr, B; Burr, F - Genetics; (United States)

We have examined insertions of the controlling element Ds at the Shrunken locus of maize. A cDNA probe complementary to a portion of the Shrunken locus mRNA was prepared. This probe recognizes a unique sequence in maize DNA. Using lines carrying derivatives of the same short arm of chromosome 9, we have detected modifications at the nucleic acid level caused by Ds. The changes appear to be large insertions, one of which may be more than 20 kilobase pairs in length. These observations provide a basis for the isolation and molecular characterization of one of the maize controlling elements.
Transposable elements and genetic instabilities in crop plants

Conference Burr, B; Burr, F

Transposable elements have long been associated with certain unstable loci in maize and have been intensively studied by McClintock and others. It is known that a transposable element can control the expression of the structural genes at the locus where it resides. These controlling elements in maize are now beginning to be studied at the molecular level. Using recombinant molecular probes we have been able to describe the changes induced by the controlling element Ds at the shrunken locus. Ds elements appear to be large and dissimilar insertions into the wild-type locus - two elements actually map within the transcribedmore »« less
Full Text Available
Transposable elements and genetic instabilities in crop plants

Journal Article Burr, B; Burr, F - Stadler Genet. Symp.; (United States)

Transposable elements have long been associated with certain unstable loci in maize and have been intensively studied by McClintock and others. It is known that a transposable element can control the expression of the structural genes at the locus where it resides. These controlling elements in maize are now beginning to be studied at the molecular level. Using recombinant molecular probes we have been able to describe the changes induced by the controlling element Ds at the shrunken locus. Ds elements appear to be large and dissimilar insertions into the wild-type locus - two elements actually map within the transcribedmore »« less

Similar Records