Rb and p53 gene deletions in lung adenocarcinomas from irradiated and control mice

Zhang, Y; Woloschak, G E

doi:10.2172/515531

Title: Rb and p53 gene deletions in lung adenocarcinomas from irradiated and control mice

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Abstract

This study was conducted on mouse lung adenocarcinoma tissues that were formalin-treated and paraffin-embedded 25 years ago to investigate the large gene deletions of mRb and p53 in B6CF{sub 1} male mice. A total of 80 lung tissue samples from irradiated mice and 40 lung samples from nonirradiated controls were randomly selected and examined in the mRb portion of this study. The results showed a significant (P < 0.05) higher percentage of mRb deletions in lung adenocarcinomas from mice exposed to 60 once-weekly {gamma}-ray doses than those from mice receiving 24 once-weekly {gamma}-ray doses at low doses and low dose rates; however, the percentage was not significantly different (P > 0.05) from that for spontaneous lung adenocarcinomas or lung adenocarcinomas from mice exposed to single-dose {gamma} irradiation at a similar total dose. mRb fragments 3 (71%) and 5 (67%), the parts of the gene that encoded the pocket binding region of Rb protein to adenovirus E1A and SV40 T-antigen, were the most frequently deleted fragments. p53 gene deletion analysis was carried out on normal lungs and lung adenocarcinomas that were initially found to bear mRb deletions. Exons 1,4,5,6, and 9 were chosen to be analyzed.

Authors:

Zhang, Y; Woloschak, G E ^[1]

Argonne National Lab., IL (United States). Center for Mechanistic Biology and Biotechnology

Publication Date:: Fri Aug 01 00:00:00 EDT 1997

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE Office of Energy Research, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)

OSTI Identifier:: 515531

Report Number(s):: ANL/CMB/PP-90330
ON: DE97008257; CNN: Grant ES 07141-02; TRN: 97:016585

DOE Contract Number:: W-31109-ENG-38

Resource Type:: Technical Report

Resource Relation:: Other Information: PBD: [1997]

Country of Publication:: United States

Language:: English

Subject:: 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CARCINOMAS; RADIOINDUCTION; LUNGS; BIOLOGICAL MODELS; EXPERIMENTAL DATA; MUTAGENESIS; GAMMA RADIATION; EXONS

Citation Formats


                    Zhang, Y, and Woloschak, G E. Rb and p53 gene deletions in lung adenocarcinomas from irradiated and control mice.  United States: N. p., 1997. 
        Web.  doi:10.2172/515531.

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                    Zhang, Y, & Woloschak, G E. Rb and p53 gene deletions in lung adenocarcinomas from irradiated and control mice.  United States.  https://doi.org/10.2172/515531

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                    Zhang, Y, and Woloschak, G E. 1997.  
        "Rb and p53 gene deletions in lung adenocarcinomas from irradiated and control mice".  United States.  https://doi.org/10.2172/515531.  https://www.osti.gov/servlets/purl/515531.

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@article{osti_515531,

  title        = {Rb and p53 gene deletions in lung adenocarcinomas from irradiated and control mice},

  author       = {Zhang, Y and Woloschak, G E},

  abstractNote = {This study was conducted on mouse lung adenocarcinoma tissues that were formalin-treated and paraffin-embedded 25 years ago to investigate the large gene deletions of mRb and p53 in B6CF{sub 1} male mice. A total of 80 lung tissue samples from irradiated mice and 40 lung samples from nonirradiated controls were randomly selected and examined in the mRb portion of this study. The results showed a significant (P < 0.05) higher percentage of mRb deletions in lung adenocarcinomas from mice exposed to 60 once-weekly {gamma}-ray doses than those from mice receiving 24 once-weekly {gamma}-ray doses at low doses and low dose rates; however, the percentage was not significantly different (P > 0.05) from that for spontaneous lung adenocarcinomas or lung adenocarcinomas from mice exposed to single-dose {gamma} irradiation at a similar total dose. mRb fragments 3 (71%) and 5 (67%), the parts of the gene that encoded the pocket binding region of Rb protein to adenovirus E1A and SV40 T-antigen, were the most frequently deleted fragments. p53 gene deletion analysis was carried out on normal lungs and lung adenocarcinomas that were initially found to bear mRb deletions. Exons 1,4,5,6, and 9 were chosen to be analyzed.},

  doi          = {10.2172/515531},

  url          = {https://www.osti.gov/biblio/515531},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Aug 01 00:00:00 EDT 1997},

  month        = {Fri Aug 01 00:00:00 EDT 1997}

}

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