Oncogene amplification detected by in situ hybridization in radiation induced rat skin tumors. [C-myc:a3]

Technical Report · Fri Feb 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/5106315· OSTI ID:5106315

Yi, Jin

Oncogene activation may play an important role in radiation induced carcinogenesis. C-myc oncogene amplification was detected by in situ hybridization in radiation-induced rat skin tumors, including squamous and basal cell carcinomas. In situ hybridization was performed with a biotinylated human c-myc third exon probe, visualized with an avidin-biotinylated alkaline phosphate detection system. No c-myc oncogene amplification was detected in normal rat skin at very early times after exposure to ionizing radiation, which is consistent with the view that c-myc amplification is more likely to be related to carcinogenesis than to normal cell proliferation. The incorporation of tritiated thymidine into the DNA of rat skin cells showed that the proliferation of epidermal cells reached a peak on the seventh day after exposure to ionizing radiation and then decreased. No connection between the proliferation of epidermal cell and c-myc oncogene amplification in normal or irradiated rat skin was found. The results indicated that c-myc amplification as measured by in situ hybridization was correlated with the Southern bolt results, but only some of the cancer cells were amplified. The c-myc positive cells were distributed randomly within regions of the tumor and exhibited a more uniform nuclear structure in comparison to the more vacuolated c-myc negative cells. No c-myc signal was detected in unirradiated normal skin or in irradiated skin cells near the tumors. C-myc amplification appears to be cell or cell cycle specific within radiation-induced carcinomas. 28 refs., 3 figs., 3 tabs.

• Research Organization: New York Univ., NY (United States). Dept. of Environmental Medicine
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-87ER60539
• OSTI ID: 5106315
• Report Number(s): DOE/ER/60539-8; ON: DE92002475
• Resource Relation: Other Information: Thesis (M.S.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CARCINOGENESIS
BIOLOGICAL PATHWAYS
NEOPLASMS
RADIOINDUCTION
ONCOGENES
GENE AMPLIFICATION
GENE REGULATION
SKIN
BIOLOGICAL RADIATION EFFECTS
CELL PROLIFERATION
DNA HYBRIDIZATION
ELECTRON BEAMS
HISTOLOGICAL TECHNIQUES
IONIZING RADIATIONS
ONCOGENIC TRANSFORMATIONS
RATS
ANIMALS
BEAMS
BIOLOGICAL EFFECTS
BODY
CELL TRANSFORMATIONS
DISEASES
GENES
HYBRIDIZATION
LEPTON BEAMS
MAMMALS
ORGANS
PARTICLE BEAMS
PATHOGENESIS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550400 - Genetics
550300 - Cytology
550200 - Biochemistry