Molecular biological enhancement of coal desulfurization. [Thiobacillus cuprinus]

Technical Report · Wed Mar 13 00:00:00 EST 1991

DOI:https://doi.org/10.2172/5100761· OSTI ID:5100761

Litchfield, J. H.; Zupancic, T. J.; Kittle, Jr., J. D.; Baker, B.; Palmer, D. T.; Traunero, C. G.; Wyza, R. E.; Schweitzer, A.; Conkle, H. N. ^[1]; Chakravarty, L.; Tuovinen, O. H. ^[2]

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1. Battelle, Columbus, OH (United States)
2. Ohio State Univ., Columbus, OH (United States)

The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the 4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of the organism; transfer this pathway into a fast-growing chemolithotrophic bacterium; conduct a batch-mode optimization/analysis of scale-up variables.

• Research Organization: Battelle Memorial Inst., Columbus, OH (United States); The Ohio State Univ., Columbus, OH (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC22-89PC89902
• OSTI ID: 5100761
• Report Number(s): DOE/PC/89902-T12; ON: DE92015659
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
59 BASIC BIOLOGICAL SCIENCES
COAL
DESULFURIZATION
MICROORGANISMS
ORGANIC SULFUR COMPOUNDS
BIODEGRADATION
CLONING
ENZYMES
GENETIC ENGINEERING
PLASMIDS
PROGRESS REPORT
SULFATES
SULFONATES
SULFONES
SULFOXIDES
THIOBACILLUS FERROXIDANS
BACILLUS
BACTERIA
BIOTECHNOLOGY
CARBONACEOUS MATERIALS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DOCUMENT TYPES
ENERGY SOURCES
FOSSIL FUELS
FUELS
MATERIALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
SULFUR COMPOUNDS
SULFUR-OXIDIZING BACTERIA
010402* - Coal
Lignite
& Peat- Purification & Upgrading
550700 - Microbiology
550400 - Genetics