Control of mammalian cell mutagenesis and differentiation by chemicals which initiate or promote tumor formation
A cell-mediated mutagenesis assay was developed to predict the potential carcinogenic hazard of some environmental chemicals. In this assay, Chinese hamster V79 cells, which are susceptible to mutagenesis, are co-cultivated with cells capable of metabolizing chemical carcinogens. Use of this assay made it possible to demonstrate a relationship between the degree of carcinogenicity and mutagenicity of a series of polycyclic hydrocarbons and nitrosamines and to study the organ specificity exhibited by some chemical carcinogens. However, most short-term in vitro assays are designed to detect mutagenic activity and therefore do not detect tumor promoting agents which are devoid of this activity. By analyzing various markers of terminal differentiation in cultured human melanoma and myeloid leukemia cells, we have established a relationship between the activity of a series of tumor promoting phorbol diesters in the mouse skin and their ability to induce terminal differentiation. We suggest that measuring alterations in the differentiation characteristics of some cultured cells may represent an approach by which environmental tumor promoting agents can be studied and detected.
- Research Organization:
- Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences; Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5059329
- Report Number(s):
- CONF-8006117-1
- Resource Relation:
- Conference: Workshop on biological carcinogenesis, Detroit, MI, USA, 11 Jun 1980
- Country of Publication:
- United States
- Language:
- English
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Induction of mutagenesis and alterations in gene expression by tumorigenic chemicals
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Related Subjects
MUTAGEN SCREENING
TUMOR PROMOTERS
BIOASSAY
ANIMALS
AROMATICS
CARCINOGENS
ESTERS
HYDROCARBONS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RODENTS
SCREENING
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)