Capillary liquid chromatography using laser-based and mass spectrometric detection

Technical Report · Tue Jan 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/5055092· OSTI ID:5055092

Sepaniak, M J; Cook, K D

The research performed during the past year has mainly focused on investigating and minimizing the problems listed below that limit the practical utility of these capillary electrokinetic separation techniques in chemical analysis. (1) Analyses are hindered by poor reproducibility. This is largely a result of complicated and irreproducible capillary wall-solute interactions that often result in adsorption and mobility changes. (2) While the Micellar Electrokinetic Capillary Chromatography technique permits the separations of neutral solutes, hydrophobic compounds are difficult to separate and manipulation of capacity factors is critically important due to a limited elution range. Because of the limited elution range, it is also beneficial to enhance separation selectivity through the use of non-traditional surfactants. (3) The very small solute band volumes require that on-column'' detection be performed (usually optical detection) and this seriously limits detectability. Laser fluorimetry is particularly amenable to on- column detection with these capillary separation technique. We have explored methods of on-column labeling and multi-wavelength detection to expand the utility of this mode of detection. 35 refs., 7 figs.

• Research Organization: Tennessee Univ., Knoxville, TN (United States). Dept. of Chemistry
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-86ER13613
• OSTI ID: 5055092
• Report Number(s): DOE/ER/13613-31; ON: DE92002428
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ATOMIC IONS
SEPARATION PROCESSES
ENANTIOMORPHS
MICELLAR SYSTEMS
ELECTROPHORESIS
CHROMATOGRAPHY
DEXTRIN
FIBER OPTICS
FLUORESCENCE SPECTROSCOPY
LASER RADIATION
PROGRESS REPORT
SURFACTANTS
CARBOHYDRATES
CHARGED PARTICLES
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
IONS
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIATIONS
SACCHARIDES
SPECTROSCOPY
400105* - Separation Procedures