Applications of carbon-13 and sodium-23 NMR in the study of plants, animal, and human cells
Carbon-13 and sodium-23 NMR have been applied to the study of a variety of plant, animal and human cell types. Sodium NMR, in combination with dysprosium shift reagents, has been used to monitor sodium transport kinetics in salt-adapted, and non-adapted cells of P. milliaceum and whole D. spicata plants. The sodium content of human erythrocytes and leukemic macrophages was measured. Carbon-13 NMR was used to determine the structure and metabolism of rat epididymal fat pad adipocytes in real time. Insulin and isoproterenol-stimulated triacylglycerol turnover could be monitored in fat cell suspensions. (1-/sup 13/C) glucose was used as a substrate to demonstrate futile metabolic cycling from glucose to glycerol during lypolysis. Cell wall polysaccharide synthesis was followed in suspensions of P. milliaceum cells using (1-/sup 13/C) glucose as a precursor. These results illustrate the wide range of living systems which are amenable to study with NMR. 14 refs., 21 figs.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5043534
- Report Number(s):
- LA-UR-85-3447; CONF-8408176-1; ON: DE86000800
- Resource Relation:
- Conference: NATO advanced study institute, Palermo, Sicily, 1 Aug 1984
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
CELLULOSE
BIOSYNTHESIS
SODIUM
MEMBRANE TRANSPORT
TRIGLYCERIDES
METABOLISM
CARBON 13
DIABETES MELLITUS
DYSPROSIUM PHOSPHATES
ERYTHROCYTES
EXPERIMENTAL DATA
FAT CELLS
GLUCOSE
LABELLED POOL TECHNIQUES
NUCLEAR MAGNETIC RESONANCE
PH VALUE
SODIUM 23
SPIN-LATTICE RELAXATION
SYMPATHOMIMETICS
ALDEHYDES
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMAL CELLS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBON ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DISEASES
DRUGS
DYSPROSIUM COMPOUNDS
ELEMENTS
ENDOCRINE DISEASES
ESTERS
EVEN-ODD NUCLEI
HEXOSES
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MATERIALS
METABOLIC DISEASES
METALS
MONOSACCHARIDES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
POLYSACCHARIDES
RARE EARTH COMPOUNDS
RELAXATION
RESONANCE
SACCHARIDES
SODIUM ISOTOPES
SOMATIC CELLS
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
550501* - Metabolism- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics