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Title: Single-cell bioluminescence and GFP in biofilm research

Conference ·
OSTI ID:486591
 [1]
  1. Oak Ridge National Lab., TN (United States), Environmental Sciences Div.

Using flow cells and a combination of microscopy techniques, we can unequivocally identify single bacterial cells that express bioluminescent and fluorescent bioreporters. We have shown that, for attached cells, bioluminescence output within a bacterial strain can vary greatly from cell to cell.

Research Organization:
Lockheed Martin Energy Research, Inc., Oak Ridge, TN (United States); Tennessee Univ., Knoxville, TN (United States)
Sponsoring Organization:
USDOE Office of Energy Research, Washington, DC (United States); Office of Naval Research, Washington, DC (United States); National Inst. for Global Environmental Change, Davis, CA (United States)
DOE Contract Number:
FG02-96ER62163; AC05-96OR22464
OSTI ID:
486591
Report Number(s):
CONF-9610269-1; ON: DE97004284; CNN: ONR Grant N0014-1-1317; ONR Grant N00014-94-1-0441; ONR Grant N00014-94-1-0765; ONR Contract N0014-93-0326; NIGEC Grant 94UOT001S
Resource Relation:
Conference: 9. international symposium in bioluminescence and chemluminescance, Wood Hole, MA (United States), 4-8 Oct 1996; Other Information: PBD: 1996
Country of Publication:
United States
Language:
English