Preparation of oligonucleotide arrays for hybridization studies: Final report, 2/15/92-5/4/96
We have developed several novel ways to prepare DNA. In each, the deprotection step in each synthesis cycle is accomplished with light. The group we developed for this process is dimethoxybenzoin (DMB) which, when attached to acidic functionalities, is readily removed with long wavelength (350nm) UV irradiation that will not damage the DNA bases.
- Research Organization:
- Duke Univ., Durham, NC (United States)
- Sponsoring Organization:
- USDOE Office of Energy Research, Washington, DC (United States)
- DOE Contract Number:
- FG05-92ER61388
- OSTI ID:
- 486589
- Report Number(s):
- DOE/ER/61388-T1; ON: DE97003638
- Resource Relation:
- Other Information: PBD: 1996
- Country of Publication:
- United States
- Language:
- English
Similar Records
Synthesis of polycyclic aromatic hydrocarbon adducts of adenine in nucleosides and oligonucleotides
Chimeric Proteins to Detect DNA Damage and Mismatches
Inverse phosphotriester DNA synthesis using photochemically-removable dimethoxybenzoin phosphate protecting groups
Miscellaneous
·
Wed Jan 01 00:00:00 EST 1992
·
OSTI ID:486589
Chimeric Proteins to Detect DNA Damage and Mismatches
Technical Report
·
Mon Jan 14 00:00:00 EST 2002
·
OSTI ID:486589
Inverse phosphotriester DNA synthesis using photochemically-removable dimethoxybenzoin phosphate protecting groups
Journal Article
·
Fri Mar 22 00:00:00 EST 1996
· Journal of Organic Chemistry
·
OSTI ID:486589
+1 more