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Title: Controlled production of cellulases in plants for biomass conversion. Progress report, June 15, 1996--March 10, 1997

Abstract

The goal of this project is to facilitate conversion of plant biomass to usable energy by developing transgenic plants that express genes for microbial cellulases, which can be activated after harvest of the plants. In particular, we want to determine the feasibility of targeting an endoglucanase and a cellobiohydrolase to the plant apoplast (cell wall milieu). The apoplast not only contains cellulose, the substrate for the enzymes, but also can tolerate large amounts of foreign protein. To avoid detrimental effects of cellulase expression in plants, we have chosen enzymes with high temperature optima; the genes for these enzymes are from thermophilic organisms that can use cellulose as a sole energy source.

Authors:
Publication Date:
Research Org.:
Colorado Univ., Boulder, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Research, Washington, DC (United States)
OSTI Identifier:
481937
Report Number(s):
DOE/ER/12194-T1
ON: DE97006380; TRN: 97:003660
DOE Contract Number:  
FG03-96ER12194
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1997]
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; CELLULASE; ENZYME ACTIVITY; BIOMASS; CONVERSION; PROGRESS REPORT; CELL WALL; CELLULOSE; TRANSGENIC PLANTS; GENETIC ENGINEERING; PLASMIDS; ARABIDOPSIS

Citation Formats

Danna, K J. Controlled production of cellulases in plants for biomass conversion. Progress report, June 15, 1996--March 10, 1997. United States: N. p., 1997. Web. doi:10.2172/481937.
Danna, K J. Controlled production of cellulases in plants for biomass conversion. Progress report, June 15, 1996--March 10, 1997. United States. https://doi.org/10.2172/481937
Danna, K J. 1997. "Controlled production of cellulases in plants for biomass conversion. Progress report, June 15, 1996--March 10, 1997". United States. https://doi.org/10.2172/481937. https://www.osti.gov/servlets/purl/481937.
@article{osti_481937,
title = {Controlled production of cellulases in plants for biomass conversion. Progress report, June 15, 1996--March 10, 1997},
author = {Danna, K J},
abstractNote = {The goal of this project is to facilitate conversion of plant biomass to usable energy by developing transgenic plants that express genes for microbial cellulases, which can be activated after harvest of the plants. In particular, we want to determine the feasibility of targeting an endoglucanase and a cellobiohydrolase to the plant apoplast (cell wall milieu). The apoplast not only contains cellulose, the substrate for the enzymes, but also can tolerate large amounts of foreign protein. To avoid detrimental effects of cellulase expression in plants, we have chosen enzymes with high temperature optima; the genes for these enzymes are from thermophilic organisms that can use cellulose as a sole energy source.},
doi = {10.2172/481937},
url = {https://www.osti.gov/biblio/481937}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jun 01 00:00:00 EDT 1997},
month = {Sun Jun 01 00:00:00 EDT 1997}
}