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Title: Genomic plasticity and catabolic potential of Pseudomonas cepacia

Abstract

The primary goal of this project was to gain information about the size and organization of the genome of Burkholderia cepacia (formerly Pseudomonas cepacia), a microbe which continues to attract attention because of its extraordinary degradative abilities and potential as an agent of bioremediation. This bacterium is no longer considered to be a member of genus Pseudomonas nor does it belong in the gamma-subclass of the proteobacteria, in which the authentic pseudomonads are grouped. It belongs in the less well characterized beta-subclass of the proteobacteria. Technology for manipulation of large DNA fragments developed by Cantor was used to demonstrate that chromosomal multiplicity, a characteristic yet to be observed in a gamma-subclass bacterium, is common among B. cepacia strains. A derivative of Tn5 suitable for determining the chromosomal locations of various B. cepacia genes was also constructed.

Authors:
Publication Date:
Research Org.:
Massachusetts Univ., Amherst, MA (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
224251
Report Number(s):
DOE/ER/20051-T1
ON: DE96009710; TRN: 96:002713
DOE Contract Number:  
FG02-91ER20051
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1996]
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; VARIATIONS; PSEUDOMONAS

Citation Formats

Lessie, T G. Genomic plasticity and catabolic potential of Pseudomonas cepacia. United States: N. p., 1996. Web. doi:10.2172/224251.
Lessie, T G. Genomic plasticity and catabolic potential of Pseudomonas cepacia. United States. https://doi.org/10.2172/224251
Lessie, T G. 1996. "Genomic plasticity and catabolic potential of Pseudomonas cepacia". United States. https://doi.org/10.2172/224251. https://www.osti.gov/servlets/purl/224251.
@article{osti_224251,
title = {Genomic plasticity and catabolic potential of Pseudomonas cepacia},
author = {Lessie, T G},
abstractNote = {The primary goal of this project was to gain information about the size and organization of the genome of Burkholderia cepacia (formerly Pseudomonas cepacia), a microbe which continues to attract attention because of its extraordinary degradative abilities and potential as an agent of bioremediation. This bacterium is no longer considered to be a member of genus Pseudomonas nor does it belong in the gamma-subclass of the proteobacteria, in which the authentic pseudomonads are grouped. It belongs in the less well characterized beta-subclass of the proteobacteria. Technology for manipulation of large DNA fragments developed by Cantor was used to demonstrate that chromosomal multiplicity, a characteristic yet to be observed in a gamma-subclass bacterium, is common among B. cepacia strains. A derivative of Tn5 suitable for determining the chromosomal locations of various B. cepacia genes was also constructed.},
doi = {10.2172/224251},
url = {https://www.osti.gov/biblio/224251}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Apr 01 00:00:00 EST 1996},
month = {Mon Apr 01 00:00:00 EST 1996}
}