NMR solution structures of adducts derived from the binding of polycyclic aromatic diol epoxides to DNA

Conference · Sun Dec 31 00:00:00 EST 1995

OSTI ID:219313

Cosman, M; Patel, D J ^[1]; Hingerty, B E ^[2]; Amin, S ^[3]; Broyde, S; Geacintov, N E ^[4]

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1. Memorial Sloan Kettering Cancer Center, New York, NY (United States). Cellular Biochemistry and Biophysics Program
2. Oak Ridge National Lab., TN (United States). Health and Safety Research Div.
3. American Health Foundation, Valhalla, NY (United States)
4. New York Univ., NY (United States)

Site-specifically modified oligonucleotides were derived from the reactions of stereoisomeric polycyclic aromatic diol epoxide metabolite model compounds with oligonucleotides of defined base composition and sequence. The NMR solution structures of ten different adducts studied so far are briefly described, and it is shown that stereochemical factors and the nature of the oligonucleotide context of the complementary strands, exert a powerful influence on the conformational features of these adducts.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); New York Univ., NY (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
• DOE Contract Number: AC05-96OR22464; FG02-86ER60405; FG02-90ER60931
• OSTI ID: 219313
• Report Number(s): CONF-9509344-1; ON: DE96009719; CNN: Grant CA 46533; Grant CA 20851; Grant CA 28038; TRN: AHC29609%%44
• Resource Relation: Conference: 15. international symposium on polycyclic aromatic hydrocarbons, Belgirate (Italy), 19-22 Sep 1995; Other Information: PBD: [1995]
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA ADDUCTS
MOLECULAR STRUCTURE
EPOXIDES
BIOCHEMICAL REACTION KINETICS
MOLECULAR BIOLOGY
NMR SPECTRA
DATA