skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Modeling AFM Induced Mechanical Deformation of Living Cells

Abstract

Finite element modeling has been applied to study deformation of living cells in Atomic Force Microscopy (AFM) and particularly Recognition Force Microscopy (RFM). The abstract mechanical problem of interest is the response to RFM point loads of an incompressible medium enclosed in a fluid membrane. Cells are soft systems, susceptible to large deformations in the course of an RFM measurement. Often the local properties such as receptor anchoring forces, the reason for the measurement, are obscured by the response of the cell as a whole. Modeling can deconvolute these effects. This facilitates experimental efforts to have reproducible measurements of mechanical and chemical properties at specific kinds of receptor sites on the membrane of a living cell. In this article we briefly review the RFM technique for cells and the problems it poses, and then report on recent progress in modeling the deformation of cells by a point load.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
15002269
Report Number(s):
UCRL-JC-151068
TRN: US200410%%58
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: International Conference on Computational Nanoscience 2003, San Francisco, CA (US), 02/23/2003--02/27/2003; Other Information: PBD: 15 Nov 2002
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; ATOMIC FORCE MICROSCOPY; CHEMICAL PROPERTIES; DEFORMATION; FASTENING; MEMBRANES; MICROSCOPY; SIMULATION

Citation Formats

Rudd, R E, McElfresh, M, Balhorn, R, Allen, M J, and Belak, J. Modeling AFM Induced Mechanical Deformation of Living Cells. United States: N. p., 2002. Web.
Rudd, R E, McElfresh, M, Balhorn, R, Allen, M J, & Belak, J. Modeling AFM Induced Mechanical Deformation of Living Cells. United States.
Rudd, R E, McElfresh, M, Balhorn, R, Allen, M J, and Belak, J. 2002. "Modeling AFM Induced Mechanical Deformation of Living Cells". United States. https://www.osti.gov/servlets/purl/15002269.
@article{osti_15002269,
title = {Modeling AFM Induced Mechanical Deformation of Living Cells},
author = {Rudd, R E and McElfresh, M and Balhorn, R and Allen, M J and Belak, J},
abstractNote = {Finite element modeling has been applied to study deformation of living cells in Atomic Force Microscopy (AFM) and particularly Recognition Force Microscopy (RFM). The abstract mechanical problem of interest is the response to RFM point loads of an incompressible medium enclosed in a fluid membrane. Cells are soft systems, susceptible to large deformations in the course of an RFM measurement. Often the local properties such as receptor anchoring forces, the reason for the measurement, are obscured by the response of the cell as a whole. Modeling can deconvolute these effects. This facilitates experimental efforts to have reproducible measurements of mechanical and chemical properties at specific kinds of receptor sites on the membrane of a living cell. In this article we briefly review the RFM technique for cells and the problems it poses, and then report on recent progress in modeling the deformation of cells by a point load.},
doi = {},
url = {https://www.osti.gov/biblio/15002269}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Nov 15 00:00:00 EST 2002},
month = {Fri Nov 15 00:00:00 EST 2002}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: