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Title: A Genetic Screen for Mutations Affecting Cell Division in the Arabidopsis thaliana Embryo Identifies Seven Loci Required for Cytokinesis

Journal Article · · PLoS ONE
 [1];  [1];  [2];  [1];  [2]
  1. Carnegie Inst., Stanford, CA (United States). Dept. of Plant Biology; Stanford Univ., CA (United States). Dept. of Biological Sciences
  2. Carnegie Inst., Stanford, CA (United States). Dept. of Plant Biology
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Cytokinesis in plants involves the formation of unique cellular structures such as the phragmoplast and the cell plate, both of which are required to divide the cell after nuclear division. In order to isolate genes that are involved in de novo cell wall formation, we performed a large-scale, microscope-based screen for Arabidopsis mutants that severely impair cytokinesis in the embryo. We recovered 35 mutations that form abnormally enlarged cells with multiple, often polyploid nuclei and incomplete cell walls. These mutants represent seven genes, four of which have previously been implicated in phragmoplast or cell plate function. Mutations in two loci show strongly reduced transmission through the haploid gametophytic generation. Molecular cloning of both corresponding genes reveals that one is represented by hypomorphic alleles of the kinesin-5 gene RADIALLY SWOLLEN 7 (homologous to tobacco kinesin-related protein TKRP125), and that the other gene corresponds to the Arabidopsis FUSED ortholog TWO-IN-ONE (originally identified based on its function in pollen development). No mutations that completely abolish the formation of cross walls in diploid cells were found. Lastly, our results support the idea that cytokinesis in the diploid and haploid generations involve similar mechanisms.

Research Organization:
Carnegie Inst. of Washington, Stanford, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
FG02-03ER20133; FG02-94ER20133
OSTI ID:
1393410
Journal Information:
PLoS ONE, Vol. 11, Issue 1; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 13 works
Citation information provided by
Web of Science

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Bulked-Segregant Analysis Coupled to Whole Genome Sequencing (BSA-Seq) for Rapid Gene Cloning in Maize journal September 2018
Bulked-Segregant Analysis Coupled to Whole Genome Sequencing (BSA-Seq) for Rapid Gene Cloning in Maize posted_content June 2018
Genome‐wide identification of EMBRYO ‐ DEFECTIVE ( EMB ) genes required for growth and development in Arabidopsis journal September 2019
A Single Nucleotide Deletion in an ABC Transporter Gene Leads to a Dwarf Phenotype in Watermelon journal November 2019
NtKRP, a kinesin-12 protein, regulates embryo/seed size and seed germination via involving in cell cycle progression at the G2/M transition journal October 2016
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Related Subjects

60 APPLIED LIFE SCIENCES
Embryos
Seedlings
Cytokinesis
Arabidopsis thailana
Alleles
cell cycle and cell division
Pollen
Mutation