Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Ward, Thomas E.

Title: Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

Patent · Tue Aug 15 00:00:00 EDT 2017

OSTI ID:1375233

Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Ward, Thomas E.

A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.

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Research Organization:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC07-99ID13727; AC07-05ID14517

Assignee:: Battelle Energy Alliance, LLC

Patent Number(s):: 9,732,330

Application Number:: 15/074,297

OSTI ID:: 1375233

Resource Relation:: Patent File Date: 2016 Mar 18

Country of Publication:: United States

Language:: English

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Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates Řezáčová, Pavlína; Kožíšek, Milan; Moy, Shiu F. Molecular Microbiology, Vol. 69, Issue 4, p. 895-910 https://doi.org/10.1111/j.1365-2958.2008.06318.x	journal	August 2008

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