Methods of introducing nucleic acids into cellular DNA

Lajoie, Marc J.; Gregg, Christopher J.; Mosberg, Joshua A.; Church, George M.

Title: Methods of introducing nucleic acids into cellular DNA

Patent · Tue Jun 27 00:00:00 EDT 2017

OSTI ID:1366739

Lajoie, Marc J.; Gregg, Christopher J.; Mosberg, Joshua A.; Church, George M.

A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.

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Research Organization:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-02ER63445

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: 9,688,994

Application Number:: 13/954,351

OSTI ID:: 1366739

Resource Relation:: Patent File Date: 2013 Jul 30

Country of Publication:: United States

Language:: English

References (25)

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