Root bacterial endophytes alter plant phenotype, but not physiology

Henning, Jeremiah A.; Weston, David J.; Pelletier, Dale A.; Timm, Collin M.; Jawdy, Sara S.; Classen, Aimée T.

doi:10.7717/peerj.2606

Title: Root bacterial endophytes alter plant phenotype, but not physiology

Journal Article · Tue Nov 01 00:00:00 EDT 2016 · PeerJ

DOI:https://doi.org/10.7717/peerj.2606· OSTI ID:1356906

Henning, Jeremiah A. ^[1]; Weston, David J. ^[2]; Pelletier, Dale A. ^[2];

^[1]; Jawdy, Sara S. ^[2]; Classen, Aimée T. ^[3]

Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States); Univ. of Copenhagen, Copenhagen (Denmark)

Plant traits, such as root and leaf area, influence how plants interact with their environment and the diverse microbiota living within plants can influence plant morphology and physiology. Here, we explored how three bacterial strains isolated from thePopulusroot microbiome, influenced plant phenotype. We chose three bacterial strains that differed in predicted metabolic capabilities, plant hormone production and metabolism, and secondary metabolite synthesis. We inoculated each bacterial strain on a single genotype ofPopulus trichocarpaand measured the response of plant growth related traits (root:shoot, biomass production, root and leaf growth rates) and physiological traits (chlorophyll content, net photosynthesis, net photosynthesis at saturating light–A_sat, and saturating CO₂–A_max). Overall, we found that bacterial root endophyte infection increased root growth rate up to 184% and leaf growth rate up to 137% relative to non-inoculated control plants, evidence that plants respond to bacteria by modifying morphology. However, endophyte inoculation had no influence on total plant biomass and photosynthetic traits (net photosynthesis, chlorophyll content). In sum, bacterial inoculation did not significantly increase plant carbon fixation and biomass, but their presence altered where and how carbon was being allocated in the plant host.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; SC0010562

OSTI ID:: 1356906

Alternate ID(s):: OSTI ID: 1394451; OSTI ID: 1501387

Journal Information:: PeerJ, Vol. 4; ISSN 2167-8359

Publisher:: PeerJ Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 27 works

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Title: Root bacterial endophytes alter plant phenotype, but not physiology

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