Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research

Liu, Degao; Hu, Rongbin; Palla, Kaitlin J.; Tuskan, Gerald A.; Yang, Xiaohan

doi:10.1016/j.pbi.2016.01.007

Title: Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research

Journal Article · Thu Feb 18 00:00:00 EST 2016 · Current Opinion in Plant Biology

DOI:https://doi.org/10.1016/j.pbi.2016.01.007· OSTI ID:1355869

Liu, Degao ^[1]; Hu, Rongbin ^[1]; Palla, Kaitlin J. ^[1]; Tuskan, Gerald A. ^[1]; Yang, Xiaohan ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division

Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. Here, this article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, as well as identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725; SC0008834

OSTI ID:: 1355869

Alternate ID(s):: OSTI ID: 1352955

Journal Information:: Current Opinion in Plant Biology, Vol. 30, Issue C; ISSN 1369-5266

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 70 works

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