Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound

Kirby, James; Fortman, Jeffrey L.; Nishimoto, Minobu; Keasling, Jay D.

Title: Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound

Patent · Tue May 02 00:00:00 EDT 2017

OSTI ID:1354809

Kirby, James; Fortman, Jeffrey L.; Nishimoto, Minobu; Keasling, Jay D.

The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California

Patent Number(s):: 9,637,766

Application Number:: 15/186,202

OSTI ID:: 1354809

Resource Relation:: Patent File Date: 2016 Jun 17

Country of Publication:: United States

Language:: English

References (10)

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Host Cells and Methods for Producing Isoprenyl Alkanoates Lee, Taek Soon; Fortman, Jeffrey L.; Keasling, Jay D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100180491 US Patent Application 12/644531; 20100180491	patent-application	July 2010
Biosynthesis of isoprenoids via the non-mevalonate pathway Eisenreich, W.; Bacher, A.; Arigoni, D. Cellular and Molecular Life Sciences, Vol. 61, Issue 12, p. 1401-1426 https://doi.org/10.1007/s00018-004-3381-z	journal	June 2004
Computational Analysis of the Evolution of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase, an Important Enzyme in Plant Terpene Biosynthesis Fung, Pui Kwan; Krushkal, Julia; Weathers, Pamela J. Chemistry & Biodiversity, Vol. 7, Issue 5, p. 1098-1110 https://doi.org/10.1002/cbdv.200900313	journal	May 2010
Phosphorylation of 1-deoxy-D-xylulose by D-xylulokinase of Escherichia coli: Wungsintaweekul, Juraithip; Herz, Stefan; Hecht, Stefan European Journal of Biochemistry, Vol. 268, Issue 2, p. 310-316 https://doi.org/10.1046/j.1432-1033.2001.01875.x	journal	January 2001
Conversion of Methylglyoxal to Acetol by Escherichia coli Aldo-Keto Reductases Ko, J.; Kim, I.; Yoo, S. Journal of Bacteriology, Vol. 187, Issue 16, p. 5782-5789 https://doi.org/10.1128/JB.187.16.5782-5789.2005	journal	August 2005
Mutations in Escherichia coli aceE and ribB Genes Allow Survival of Strains Defective in the First Step of the Isoprenoid Biosynthesis Pathway Perez-Gil, Jordi; Uros, Eva Maria; Sauret-Güeto, Susanna PLoS ONE, Vol. 7, Issue 8, Article No. e43775 https://doi.org/10.1371/journal.pone.0043775	journal	August 2012
Mono and diterpene production in Escherichia coli Reiling, K. Kinkead; Yoshikuni, Yasuo; Martin, Vincent J.J. Biotechnology and Bioengineering, Vol. 87, Issue 2, p. 200-212 https://doi.org/10.1002/bit.20128	journal	January 2004
A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy- d -xylulose 5-phosphate synthase Sauret-Güeto, Susanna; Urós, Eva María; Ibáñez, Ester FEBS Letters, Vol. 580, Issue 3, p. 736-740 https://doi.org/10.1016/j.febslet.2005.12.092	journal	January 2006
Identification of Isopentenol Biosynthetic Genes from Bacillus subtilis by a Screening Method Based on Isoprenoid Precursor Toxicity Withers, S. T.; Gottlieb, S. S.; Lieu, B. Applied and Environmental Microbiology, Vol. 73, Issue 19, p. 6277-6283 https://doi.org/10.1128/AEM.00861-07	journal	August 2007

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