U.S. Department of EnergyOffice of Scientific and Technical Information
Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock
Abstract
A significant fraction (~ 20%) of microbial life is found in the terrestrial deep subsurface, yet the metabolic processes extant in those environments are poorly understood. Here we show that H2, injected into the Opalinus Clay formation in a borehole located 300 meters below the surface, fuels a community of microorganisms with interconnected metabolisms. Metagenomic binning and metaproteomic analysis reveal a complete carbon cycle, driven by autotrophic hydrogen oxidizers. Dead biomass from these organisms is a substrate for a fermenting bacterium that produces acetate as a product. In turn, complete oxidizer heterotrophic sulfate- reducing bacteria utilize acetate and oxidize it to CO2, closing the cycle. This metabolic reconstruction sheds light onto a hydrogen-driven carbon cycle, and a sunlight-independent ecosystem in the deep subsurface.
- Authors:
-
[3];
[4];
- Ecole Polytechnique Federale Lausanne (Switzlerland). Environmental Microbiology Lab.
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
- Bioinformatics Infrastructure for Life Sciences (BILS), Stockholm (Sweden); KTH Royal Inst. of Technology, Stockholm (Sweden). School of Biotechnology, Division of Gene Technology, Science for Life Lab.
- KTH Royal Inst. of Technology, Stockholm (Sweden). School of Biotechnology, Division of Gene Technology, Science for Life Lab.
- National Cooperative for the Disposal of Radioactive Waste (Nagra), Wettingen (Switzerland)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); Swedish Research Council (SRC)
- OSTI Identifier:
- 1352739
- Grant/Contract Number:
- AC05-00OR22725; AC02-05CH11231; CSP 1505; 2011-5689
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 10; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bagnoud, Alexandre, Chourey, Karuna, Hettich, Robert L., de Bruijn, Ino, Andersson, Anders F., Leupin, Olivier X., Schwyn, Bernhard, and Bernier-Latmani, Rizlan. Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock. United States: N. p., 2016.
Web. doi:10.1038/ncomms12770.
Bagnoud, Alexandre, Chourey, Karuna, Hettich, Robert L., de Bruijn, Ino, Andersson, Anders F., Leupin, Olivier X., Schwyn, Bernhard, & Bernier-Latmani, Rizlan. Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock. United States. https://doi.org/10.1038/ncomms12770
Bagnoud, Alexandre, Chourey, Karuna, Hettich, Robert L., de Bruijn, Ino, Andersson, Anders F., Leupin, Olivier X., Schwyn, Bernhard, and Bernier-Latmani, Rizlan. 2016.
"Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock". United States. https://doi.org/10.1038/ncomms12770. https://www.osti.gov/servlets/purl/1352739.
@article{osti_1352739,
title = {Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock},
author = {Bagnoud, Alexandre and Chourey, Karuna and Hettich, Robert L. and de Bruijn, Ino and Andersson, Anders F. and Leupin, Olivier X. and Schwyn, Bernhard and Bernier-Latmani, Rizlan},
abstractNote = {A significant fraction (~ 20%) of microbial life is found in the terrestrial deep subsurface, yet the metabolic processes extant in those environments are poorly understood. Here we show that H2, injected into the Opalinus Clay formation in a borehole located 300 meters below the surface, fuels a community of microorganisms with interconnected metabolisms. Metagenomic binning and metaproteomic analysis reveal a complete carbon cycle, driven by autotrophic hydrogen oxidizers. Dead biomass from these organisms is a substrate for a fermenting bacterium that produces acetate as a product. In turn, complete oxidizer heterotrophic sulfate- reducing bacteria utilize acetate and oxidize it to CO2, closing the cycle. This metabolic reconstruction sheds light onto a hydrogen-driven carbon cycle, and a sunlight-independent ecosystem in the deep subsurface.},
doi = {10.1038/ncomms12770},
url = {https://www.osti.gov/biblio/1352739},
journal = {Nature Communications},
issn = {2041-1723},
number = 10,
volume = 7,
place = {United States},
year = {Fri Oct 14 00:00:00 EDT 2016},
month = {Fri Oct 14 00:00:00 EDT 2016}
}
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