Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8

Zeng, Xiaoli; Ren, Zhong; Wu, Qi; Fan, Jun; Peng, Pan -Pan; Tang, Kun; Zhang, Ruiqin; Zhao, Kai -Hong; Yang, Xiaojing

doi:10.1038/nplants.2014.6

Title: Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8

Journal Article · Thu Jan 08 00:00:00 EST 2015 · Nature Plants (Online)

DOI:https://doi.org/10.1038/nplants.2014.6· OSTI ID:1347118

Zeng, Xiaoli ^[1]; Ren, Zhong ^[2]; Wu, Qi ^[3]; Fan, Jun ^[3]; Peng, Pan -Pan ^[1]; Tang, Kun ^[1]; Zhang, Ruiqin ^[3]; Zhao, Kai -Hong ^[1]; Yang, Xiaojing ^[4]

Huazhong Agricultural Univ., Hubei (People's Republic of China)
Renz Research Inc., Westmont, IL (United States)
City Univ. of Hong Kong, Kowloon (Hong Kong)
The Univ. of Chicago, Chicago, IL (United States)

Arabidopsis thaliana UVR8 (AtUVR8) is a long-sought-after photoreceptor that undergoes dimer dissociation in response to UV-B light. Crystallographic and mutational studies have identified two crucial tryptophan residues for UV-B responses in AtUVR8. However, the mechanism of UV-B perception and structural events leading up to dimer dissociation remain elusive at the molecular level. We applied dynamic crystallography to capture light-induced structural events in photoactive AtUVR8 crystals. Here we report two intermediate structures at 1.67Å resolution. At the epicenter of UV-B signaling, concerted motions associated with Trp285/Trp233 lead to ejection of a water molecule, which weakens an intricate network of hydrogen bonds and salt bridges at the dimer interface. Partial opening of the β-propeller structure due to thermal relaxation of conformational strains originating in the epicenter further disrupts the dimer interface and leads to dimer dissociation. Ultimately, these dynamic crystallographic observations provide structural insights into the photo-perception and signaling mechanism of UVR8.

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Research Organization:: UChicago, LLC., Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357; GM036452; EY024363; 31270893; 31110103912

OSTI ID:: 1347118

Journal Information:: Nature Plants (Online), Vol. 1, Issue 1; ISSN 2055-0278

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 42 works

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UVR8-dependent reporters reveal spatial characteristics of signal spreading in plant tissues Vanhaelewyn, Lucas; Bernula, Péter; Van Der Straeten, Dominique Photochemical & Photobiological Sciences, Vol. 18, Issue 5 https://doi.org/10.1039/c8pp00492g	journal	January 2019
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Title: Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8

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