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Title: Longitudinal analysis of microbiota in microalga Nannochloropsis salina cultures

Abstract

Here, large-scale open microalgae cultivation has tremendous potential to make a significant contribution to replacing petroleum-based fuels with biofuels. Open algal cultures are unavoidably inhabited with a diversity of microbes that live on, influence, and shape the fate of these ecosystems. However, there is little understanding of the resilience and stability of the microbial communities in engineered semicontinuous algal systems. To evaluate the dynamics and resilience of the microbial communities in microalgae biofuel cultures, we conducted a longitudinal study on open systems to compare the temporal profiles of the microbiota from two multigenerational algal cohorts, which include one seeded with the microbiota from an in-house culture and the other exogenously seeded with a natural-occurring consortia of bacterial species harvested from the Pacific Ocean. From these month-long, semicontinuous open microalga Nannochloropsis salina cultures, we sequenced a time-series of 46 samples, yielding 8804 operational taxonomic units derived from 9,160,076 high-quality partial 16S rRNA sequences. We provide quantitative evidence that clearly illustrates the development of microbial community is associated with microbiota ancestry. In addition, N. salina growth phases were linked with distinct changes in microbial phylotypes. Alteromonadeles dominated the community in the N. salina exponential phase whereas Alphaproteobacteria and Flavobacteriia were more prevalentmore » in the stationary phase. We also demonstrate that the N. salina-associated microbial community in open cultures is diverse, resilient, and dynamic in response to environmental perturbations. This knowledge has general implications for developing and testing design principles of cultivated algal systems.« less

Authors:
 [1];  [1];  [1];  [1];  [2]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Univ. of the Philippines, Diliman Quezon City (Philippines)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1343273
Report Number(s):
SAND-2016-1584J
Journal ID: ISSN 0095-3628; PII: 746
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Microbial Ecology
Additional Journal Information:
Journal Volume: 72; Journal Issue: 1; Journal ID: ISSN 0095-3628
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biosystem; microbiota; algae; stability

Citation Formats

Geng, Haifeng, Sale, Kenneth L., Tran-Gyamfi, Mary Bao, Lane, Todd W., and Yu, Eizadora T. Longitudinal analysis of microbiota in microalga Nannochloropsis salina cultures. United States: N. p., 2016. Web. doi:10.1007/s00248-016-0746-4.
Geng, Haifeng, Sale, Kenneth L., Tran-Gyamfi, Mary Bao, Lane, Todd W., & Yu, Eizadora T. Longitudinal analysis of microbiota in microalga Nannochloropsis salina cultures. United States. https://doi.org/10.1007/s00248-016-0746-4
Geng, Haifeng, Sale, Kenneth L., Tran-Gyamfi, Mary Bao, Lane, Todd W., and Yu, Eizadora T. 2016. "Longitudinal analysis of microbiota in microalga Nannochloropsis salina cultures". United States. https://doi.org/10.1007/s00248-016-0746-4. https://www.osti.gov/servlets/purl/1343273.
@article{osti_1343273,
title = {Longitudinal analysis of microbiota in microalga Nannochloropsis salina cultures},
author = {Geng, Haifeng and Sale, Kenneth L. and Tran-Gyamfi, Mary Bao and Lane, Todd W. and Yu, Eizadora T.},
abstractNote = {Here, large-scale open microalgae cultivation has tremendous potential to make a significant contribution to replacing petroleum-based fuels with biofuels. Open algal cultures are unavoidably inhabited with a diversity of microbes that live on, influence, and shape the fate of these ecosystems. However, there is little understanding of the resilience and stability of the microbial communities in engineered semicontinuous algal systems. To evaluate the dynamics and resilience of the microbial communities in microalgae biofuel cultures, we conducted a longitudinal study on open systems to compare the temporal profiles of the microbiota from two multigenerational algal cohorts, which include one seeded with the microbiota from an in-house culture and the other exogenously seeded with a natural-occurring consortia of bacterial species harvested from the Pacific Ocean. From these month-long, semicontinuous open microalga Nannochloropsis salina cultures, we sequenced a time-series of 46 samples, yielding 8804 operational taxonomic units derived from 9,160,076 high-quality partial 16S rRNA sequences. We provide quantitative evidence that clearly illustrates the development of microbial community is associated with microbiota ancestry. In addition, N. salina growth phases were linked with distinct changes in microbial phylotypes. Alteromonadeles dominated the community in the N. salina exponential phase whereas Alphaproteobacteria and Flavobacteriia were more prevalent in the stationary phase. We also demonstrate that the N. salina-associated microbial community in open cultures is diverse, resilient, and dynamic in response to environmental perturbations. This knowledge has general implications for developing and testing design principles of cultivated algal systems.},
doi = {10.1007/s00248-016-0746-4},
url = {https://www.osti.gov/biblio/1343273}, journal = {Microbial Ecology},
issn = {0095-3628},
number = 1,
volume = 72,
place = {United States},
year = {Tue Mar 08 00:00:00 EST 2016},
month = {Tue Mar 08 00:00:00 EST 2016}
}

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Works referencing / citing this record:

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