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Title: Long-range barcode labeling-sequencing

Patent ·
OSTI ID:1329319

Methods for sequencing single large DNA molecules by clonal multiple displacement amplification using barcoded primers. Sequences are binned based on barcode sequences and sequenced using a microdroplet-based method for sequencing large polynucleotide templates to enable assembly of haplotype-resolved complex genomes and metagenomes.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
9,469,874
Application Number:
13/653,343
OSTI ID:
1329319
Resource Relation:
Patent File Date: 2012 Oct 16
Country of Publication:
United States
Language:
English

References (1)

Comprehensive human genome amplification using multiple displacement amplification journal April 2002

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