Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wet disk milling
- National Inst. of Advanced Industrial Science and Technology (AIST), Hiroshima (Japan). Biomass Refinery Research Center
- National Inst. of Advanced Industrial Science and Technology (AIST), Hiroshima (Japan). Biomass Refinery Research Center; Kangwon National University (South Korea). Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
In this study, to reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150 °C for 60 min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. Lastly, after steam treatment at 150 °C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- AC05-00OR22725
- OSTI ID:
- 1327621
- Journal Information:
- Renewable Energy, Vol. 76; ISSN 0960-1481
- Publisher:
- ElsevierCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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