Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase

Cai, Yuanheng; Zhang, Kewei; Kim, Hoon; Hou, Guichuan; Zhang, Xuebin; Yang, Huijun; Feng, Huan; Miller, Lisa; Ralph, John; Liu, Chang -Jun

doi:10.1038/ncomms11989

Title: Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase

Journal Article · Tue Jun 28 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms11989· OSTI ID:1307422

Cai, Yuanheng ^[1]; Zhang, Kewei ^[1]; Kim, Hoon ^[2]; Hou, Guichuan ^[3]; Zhang, Xuebin ^[1]; Yang, Huijun ^[1]; Feng, Huan ^[4]; Miller, Lisa ^[1]; Ralph, John ^[2];

^[1]

Brookhaven National Lab. (BNL), Upton, NY (United States)
Univ. of Wisconsin, Madison, WI (United States)
Appalachian State Univ., Boone, NC (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States); Montclair State Univ., Montclair, NJ (United States)

Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens’ lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FC02-07ER64494; SC00112704

OSTI ID:: 1307422

Alternate ID(s):: OSTI ID: 1341631

Report Number(s):: BNL-112598-2016-JA; ncomms11989

Journal Information:: Nature Communications, Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 43 works

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