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Title: Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park

Journal Article · · BioEnergy Research
 [1];  [2];  [2];  [3];  [3];  [4]
  1. C5.6 Technologies Inc., Middleton, WI (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division.
  4. Lucigen Corporation, Middleton, WI (United States)
+ Show Author Affiliations

Geobacillus thermoglucosidasius Y4.1MC1 was isolated from a boiling spring in the lower geyser basin of Yellowstone National Park. We present this species is of interest because of its metabolic versatility. The genome consists of one circular chromosome of 3,840,330 bp and a circular plasmid of 71,617 bp with an average GC content of 44.01%. The genome is available in the GenBank database (NC_014650.1 and NC_014651.1). In addition to the expected metabolic pathways for sugars and amino acids, the Y4.1MC1 genome codes for two separate carbon monoxide utilization pathways, an aerobic oxidation pathway and an anaerobic reductive acetyl CoA (Wood-Ljungdahl) pathway. This is the first report of a nonanaerobic organism with the Wood-Ljungdahl pathway. Also, this anaerobic pathway permits the strain to utilize H2 and fix CO2 present in the hot spring environment. Y4.1MC1 and its related species may play a significant role in carbon capture and sequestration in thermophilic ecosystems and may open up new routes to produce biofuels and chemicals from CO, H2, and CO2.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE; Work for Others (WFO)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1265371
Journal Information:
BioEnergy Research, Vol. 8, Issue 3; ISSN 1939-1234
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

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Cited By (7)

Unraveling the cellulolytic and hemicellulolytic potential of two novel Streptomyces strains journal September 2018
Geobacillus yumthangensis sp. nov., a thermophilic bacterium isolated from a north-east Indian hot spring journal November 2018
Draft Genome Sequence of Parageobacillus thermoglucosidasius Strain TG4, a Hydrogenogenic Carboxydotrophic Bacterium Isolated from a Marine Sediment journal January 2019
Comparative genomic analysis of Parageobacillus thermoglucosidasius strains with distinct hydrogenogenic capacities journal December 2018
Complete genome sequences of Geobacillus sp. WCH70, a thermophilic strain isolated from wood compost journal April 2016
Complete Genome Sequence of Thermus aquaticus Y51MC23 journal October 2015
Not All That Glitters Is Gold: The Paradox of CO-dependent Hydrogenogenesis in Parageobacillus thermoglucosidasius text January 2021

Figures / Tables (8)

Table 1(p. 2)table Table 1
Table 2(p. 3)table Table 2
Table 3(p. 3)table Table 3
Fig. 1(p. 4)figure Fig. 1
Fig. 2(p. 4)figure Fig. 2
Table 4(p. 5)table Table 4
Table 5(p. 5)table Table 5
Table 6(p. 6)table Table 6

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
Carbon monoxide . Carbon fixation . Wood-Ljungdahl pathway . YellowstoneNational Park . Geobacillus thermoglucosidasius