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Title: Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres

Abstract

The dynamic behaviour of supramolecular systems is an important dimension of their potential functions. Here, we report on the use of stochastic optical reconstruction microscopy to study the molecular exchange of peptide amphiphile nanofibres, supramolecular systems known to have important biomedical functions. Solutions of nanofibres labelled with different dyes (Cy3 and Cy5) were mixed, and the distribution of dyes inserting into initially single-colour nanofibres was quantified using correlative image analysis. Our observations are consistent with an exchange mechanism involving monomers or small clusters of molecules inserting randomly into a fibre. Different exchange rates are observed within the same fibre, suggesting that local cohesive structures exist on the basis of beta-sheet discontinuous domains. The results reported here show that peptide amphiphile supramolecular systems can be dynamic and that their intermolecular interactions affect exchange patterns. Lastly, this information can be used to generate useful aggregate morphologies for improved biomedical function.

Authors:
 [1];  [2]; ORCiD logo [3];  [4];  [2];  [4]
  1. Northwestern Univ., Evanston, IL (United States); Eindhoven Univ. of Technology, Eindhoven (The Netherlands); King's College London, London (United Kingdom)
  2. Eindhoven Univ. of Technology, Eindhoven (The Netherlands)
  3. Eindhoven Univ. of Technology, Eindhoven (The Netherlands); Institute for Bioengineering of Catalonia (IBEC), Barcelona (Spain)
  4. Northwestern Univ., Evanston, IL (United States)
Publication Date:
Research Org.:
Northwestern Univ., Evanston, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); Dutch Ministry of Education, Culture and Science (Gravity program); European Research Council (ERC); Marie Curie FP7-PEOPLE-IOF program
OSTI Identifier:
1261783
Alternate Identifier(s):
OSTI ID: 1820968
Grant/Contract Number:  
FG02-00ER45810
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 99 GENERAL AND MISCELLANEOUS; cells; multivalency; dynamics; medicine; vesicles; adhesion; systems; probes; biophysical chemistry; microscopy; structural biology; Biophysical chemistry Microscopy Structural biology

Citation Formats

da Silva, Ricardo M. P., van der Zwaag, Daan, Albertazzi, Lorenzo, Lee, Sungsoo S., Meijer, E. W., and Stupp, Samuel I. Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres. United States: N. p., 2016. Web. doi:10.1038/ncomms11561.
da Silva, Ricardo M. P., van der Zwaag, Daan, Albertazzi, Lorenzo, Lee, Sungsoo S., Meijer, E. W., & Stupp, Samuel I. Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres. United States. https://doi.org/10.1038/ncomms11561
da Silva, Ricardo M. P., van der Zwaag, Daan, Albertazzi, Lorenzo, Lee, Sungsoo S., Meijer, E. W., and Stupp, Samuel I. 2016. "Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres". United States. https://doi.org/10.1038/ncomms11561. https://www.osti.gov/servlets/purl/1261783.
@article{osti_1261783,
title = {Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres},
author = {da Silva, Ricardo M. P. and van der Zwaag, Daan and Albertazzi, Lorenzo and Lee, Sungsoo S. and Meijer, E. W. and Stupp, Samuel I.},
abstractNote = {The dynamic behaviour of supramolecular systems is an important dimension of their potential functions. Here, we report on the use of stochastic optical reconstruction microscopy to study the molecular exchange of peptide amphiphile nanofibres, supramolecular systems known to have important biomedical functions. Solutions of nanofibres labelled with different dyes (Cy3 and Cy5) were mixed, and the distribution of dyes inserting into initially single-colour nanofibres was quantified using correlative image analysis. Our observations are consistent with an exchange mechanism involving monomers or small clusters of molecules inserting randomly into a fibre. Different exchange rates are observed within the same fibre, suggesting that local cohesive structures exist on the basis of beta-sheet discontinuous domains. The results reported here show that peptide amphiphile supramolecular systems can be dynamic and that their intermolecular interactions affect exchange patterns. Lastly, this information can be used to generate useful aggregate morphologies for improved biomedical function.},
doi = {10.1038/ncomms11561},
url = {https://www.osti.gov/biblio/1261783}, journal = {Nature Communications},
issn = {2041-1723},
number = 1,
volume = 7,
place = {United States},
year = {Thu May 19 00:00:00 EDT 2016},
month = {Thu May 19 00:00:00 EDT 2016}
}

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Cited by: 106 works
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Works referenced in this record:

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Shape-Dependent Targeting of Injured Blood Vessels by Peptide Amphiphile Supramolecular Nanostructures
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Self-assembly for the synthesis of functional biomaterials
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Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
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Conformational preferences of amino acids in globular proteins
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Self-Assembly of Peptide−Amphiphile Nanofibers:  The Roles of Hydrogen Bonding and Amphiphilic Packing
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Atomistic Molecular Dynamics Simulations of Peptide Amphiphile Self-Assembly into Cylindrical Nanofibers
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Pathway Selection in Peptide Amphiphile Assembly
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High-Affinity Peptide-Based Collagen Targeting Using Synthetic Phage Mimics: From Phage Display to Dendrimer Display
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Tuning Supramolecular Rigidity of Peptide Fibers through Molecular Structure
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Tunable Mechanics of Peptide Nanofiber Gels
journal, March 2010


Equilibrium Chain Exchange Kinetics of Diblock Copolymer Micelles:  Tuning and Logarithmic Relaxation
journal, June 2006


Antitumor Activity of Peptide Amphiphile Nanofiber-Encapsulated Camptothecin
journal, October 2011


Cell death versus cell survival instructed by supramolecular cohesion of nanostructures
journal, February 2014


Consequences of chirality on the dynamics of a water-soluble supramolecular polymer
journal, February 2015


Energy landscapes and functions of supramolecular systems
journal, January 2016


More dyes enter the realm of nanoscopy
journal, September 2012


Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
journal, August 2006


Spatiotemporal control and superselectivity in supramolecular polymers using multivalency
journal, July 2013


Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C beta I.
journal, August 1996


Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes
journal, September 2007


Probing Exchange Pathways in One-Dimensional Aggregates with Super-Resolution Microscopy
journal, May 2014


Super-Resolution Fluorescence Microscopy
journal, June 2009


Correlation Functions Quantify Super-Resolution Images and Estimate Apparent Clustering Due to Over-Counting
journal, February 2012


Works referencing / citing this record:

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Quantifying Guest-Host Dynamics in Supramolecular Assemblies to Analyze Their Robustness
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Tuning the matrix metalloproteinase-1 degradability of peptide amphiphile nanofibers through supramolecular engineering
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