Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate

De Filippis, Francesca; Genovese, Alessandro; Ferranti, Pasquale; Gilbert, Jack A.; Ercolini, Danilo

doi:10.1038/srep21871

Title: Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate

Journal Article · Thu Feb 25 00:00:00 EST 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep21871· OSTI ID:1258637

De Filippis, Francesca ^[1]; Genovese, Alessandro ^[1]; Ferranti, Pasquale ^[1]; Gilbert, Jack A. ^[2]; Ercolini, Danilo ^[1]

Univ. of Naples Federico II (Italy)
Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Chicago, IL (United States)

Traditional cheeses harbour complex microbial consortia that play an important role in shaping typical sensorial properties. However, the microbial metabolism is considered difficult to control. Microbial community succession and the related gene expression were analysed during ripening of a traditional Italian cheese, identifying parameters that could be modified to accelerate ripening. Afterwards, we modulated ripening conditions and observed consistent changes in microbial community structure and function. We provide concrete evidence of the essential contribution of non-starter lactic acid bacteria in ripening-related activities. An increase in the ripening temperature promoted the expression of genes related to proteolysis, lipolysis and amino acid/lipid catabolism and significantly increases the cheese maturation rate. Moreover, temperature-promoted microbial metabolisms were consistent with the metabolomic profiles of proteins and volatile organic compounds in the cheese. Finally, the results clearly indicate how processing-driven microbiome responses can be modulated in order to optimize production efficiency and product quality.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

OSTI ID:: 1258637

Journal Information:: Scientific Reports, Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 115 works

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