Microbiome associations in pigs with the best and worst clinical outcomes following co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2)

Niederwerder, Megan C.; Jaing, Crystal J.; Thissen, James B.; Cino-Ozuna, Ada Giselle; McLoughlin, Kevin S.; Rowland, Raymond R. R.

doi:10.1016/j.vetmic.2016.03.008

Title: Microbiome associations in pigs with the best and worst clinical outcomes following co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2)

Journal Article · Thu Mar 10 00:00:00 EST 2016 · Veterinary Microbiology

DOI:https://doi.org/10.1016/j.vetmic.2016.03.008· OSTI ID:1258531

Niederwerder, Megan C. ^[1]; Jaing, Crystal J. ^[2]; Thissen, James B. ^[2]; Cino-Ozuna, Ada Giselle ^[1]; McLoughlin, Kevin S. ^[2]; Rowland, Raymond R. R. ^[1]

Kansas State Univ., Manhattan, KS (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Co-infections involving porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are common and contribute to a range of polymicrobial disease syndromes in swine and on a world-wide basis. Both viruses compromise host defenses, resulting in increased susceptibility to infections by primary and secondary pathogens that can affect growth performance as well as increased morbidity and mortality. An experimental population of 95 pigs was co-infected with PRRSV and PCV2. At 70 days post-infection (dpi), 20 representative pigs were selected as having the best or worst clinical outcome based on average daily gain (ADG) and the presence of clinical disease. Moreover, the worst clinical outcome pigs had prolonged and greater levels of viremia as measured by qPCR. Serum, lung and fecal samples collected at 70 dpi were analyzed using a comprehensive DNA microarray technology, the Lawrence Livermore Microbial Detection Array, to detect over 8000 microbes. Bacterial species, such as Bacillus cereus, were detected at a higher rate in the serum of worst performing pigs. At the level of the fecal microbiome, the overall microbial diversity was lower in the worst clinical outcome group. The results reinforce the importance of pathogen load in determining clinical outcome and suggest an important role of microbial diversity as a contributing factor in disease.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344; 14ERD081

OSTI ID:: 1258531

Alternate ID(s):: OSTI ID: 1348263

Report Number(s):: LLNL-JRNL-679863

Journal Information:: Veterinary Microbiology, Vol. 188, Issue C; ISSN 0378-1135

Country of Publication:: United States

Language:: English

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Cited by: 47 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Microbiome associations in pigs with the best and worst clinical outcomes following co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2)

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